Structural characterization of a novel polysaccharide from sweet corncob that inhibits glycosylase in STZ-induced diabetic rats Structural characterization of a novel polysaccharide

文献类型： 外文期刊

第一作者： Wang, Xin

作者： Wang, Xin;Xiu, Weiye;Han, Ye;Xie, Jingnan;Ma, Yongqiang;Zhang, Kai;Zhou, Kechi

作者机构：

关键词： Sweet corncob polysaccharides; Structural characterization; Hypoglycemic effect; Inhibition of glycosylase

期刊名称：GLYCOCONJUGATE JOURNAL （ 影响因子：3.009； 五年影响因子：3.456 ）

ISSN： 0282-0080

年卷期： 2022 年 39 卷 3 期

页码：

收录情况： SCI

摘要： In the current study, we extracted a polysaccharide from sweet corncob and evaluated its hypoglycemic function. After collection in water, alcohol precipitation, and purification by DEAE-52 and Sephadex G-100 columns, we obtained a polysaccharide (SCP50) that was composed primarily of mannose and glucose (9.73:190.27), with a molecular weight of 9280.33 Da. We demonstrated that SCP50 exhibited significant inhibition of alpha-glucosidase activity, with an IC50 of 4.866 mg/mL, K-m of 1.297 x 10(-3), and V-max of 0.076 mol/L center dot min(-1)in vitro. We also observed that SCP50 markedly attenuated disaccharidase (maltase, sucrase, and lactase) activity in a rat model of T2DM. We conclude that SCP50 exerts a hypoglycemic effect via inhibition of intestinal glycosylase. These results thus provide new insight into the hypoglycemic action underlying sweet corncob polysaccharide's effects.

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