WLP3 Encodes the Ribosomal Protein L18 and Regulates Chloroplast Development in Rice

文献类型： 外文期刊

第一作者： Lu, Tao

作者： Lu, Tao;Yin, Wenjin;Zhang, Yinuo;Zhu, Chaoyu;Zhong, Qianqian;Chen, Zhengai;Ye, Hanfei;Fang, Yuan;Rao, Yuchun;Li, Sanfeng;Wang, Yuexing;Wang, Nuo;Mu, Dan

作者机构： Zhejiang Normal Univ, Coll Life Sci, 688 Yingbin Rd, Jinhua 321000, Zhejiang, Peoples R China;China Natl Rice Res Inst, State Key Lab Rice Biol & Breeding, Hangzhou 310006, Peoples R China;Anqing Normal Univ, Coll Life Sci, Anqing 246133, Anhui, Peoples R China

关键词： Rice; Ribosomal proteins; White leaf and panicle; Chloroplast development; Abiotic stresses

期刊名称：RICE （ 2022影响因子：5.5； 五年影响因子：5.8 ）

ISSN： 1939-8425

年卷期： 2023 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Plastid ribosomal proteins play a crucial role in the growth and development of plants, mainly in the gene expression and translation of key genes in chloroplasts. While some information is known about the regulatory processes of plastid ribosomal proteins in various plant species, there is limited knowledge about the underlying mechanisms in rice. In this study, ethyl methanesulfonate (EMS) mutagenesis was used to generate a new mutant called wlp3 (white leaf and panicle3), characterized by white or albino leaves and panicles, which exhibited this phenotype from the second leaf stage until tillering. Furthermore, after a certain period, the newly emerging leaves developed the same phenotype as the rice variety ZH11, while the albino leaves of wlp3 showed an incomplete chloroplast structure and significantly low chlorophyll content. A transition mutation (T to C) at position 380 was identified in the coding region of the LOC_Os03g61260 gene, resulting in the substitution of isoleucine by threonine during translation. WLP3 encodes the ribosomal L18 subunit, which is localized in the chloroplast. Complementation experiments confirmed that LOC_Os03g61260 was responsible for the albino phenotype in rice. WLP3 has high expression in the coleoptile, leaves at the three-leaf stage, and panicles at the heading stage. Compared to the wild-type (WT), wlp3 exhibited reduced chlorophyll synthesis and significantly decreased expression levels of genes associated with plastid development. Yeast two-hybrid (Y2H) analysis revealed that WLP3 interacts with other ribosomal subunits, to influence chloroplast development. These results contribute to a better understanding of the underlying molecular mechanisms of chloroplast development and plastid gene translation.

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