Self-Assembly of Porcine Parvovirus Virus-like Particles and Their Application in Serological Assay

文献类型： 外文期刊

第一作者： Gao, Yanfei

作者： Gao, Yanfei;Wang, Haiwei;Wang, Shanghui;Sun, Mingxia;Fang, Zheng;Cai, Xuehui;Tu, Yabin;Liu, Xinran

作者机构：

关键词： porcine parvovirus; virus-like particles; diagnostic; I-ELISA

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2022 年 14 卷 8 期

页码：

收录情况： SCI

摘要： Porcine parvovirus (PPV) is widely prevalent in pig farms. PPV is closely related to porcine respiratory disease complex (PRDC) and porcine circovirus disease (PCVD), which seriously threatens the healthy development of the pig industry. Although commercial antibody detection kits are available, they are expensive and unsuitable for large-scale clinical practice. Here, a soluble VP2 protein of PPV is efficiently expressed in the E. coli expression system. The VP2 protein can be self-assembled into virus-like particles (VLPs) in vitro. After multiple steps of chromatography purification, PPV-VLPs with a purity of about 95% were obtained. An indirect, enzyme-linked immunosorbent assay (I-ELISA), comparable to a commercial PPV kit, was developed based on the purified PPV-VLPs and was used to detect 487 clinical pig serum samples. The results showed that the I-ELISA is a simple, cost-effective, and efficient method for the diagnosis of clinical pig serum and plasma samples. In summary, high-purity, tag-free PPV-VLPs were prepared, and the established VLP-based I-ELISA is of great significance for the sero-monitoring of antibodies against PPV.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序