Molecular cloning, expression pattern of Trypsin gene and association analysis with growth traits in Penaeus monodon

文献类型： 外文期刊

第一作者： Li, Yun-Dong

作者： Li, Yun-Dong;Zhou, Fa-Lin;Jiang, Song;Yang, Li-Shi;Huang, Jian-Hua;Mai, Qianlin;Jiang, Shi-Gui;Li, Yun-Dong;Zhou, Fa-Lin;Yang, Qi-Bin;Jiang, Shi-Gui;Li, Yun-Dong;Chen, Xu;Jiang, Shi-Gui

作者机构：

关键词： Gene clone; Growth traits-related; Penaeus monodon; Single nucleotide polymorphism (SNP); Trypsin gene (PmTry)

期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （ 影响因子：0.6； 五年影响因子：0.6 ）

ISSN： 0792-156X

年卷期： 2022 年 74 卷

页码：

收录情况： SCI

摘要： A novel TRY homolog was cloned in Penaeus monodon by RACE technology, named PmTry (GenBank: KP998480). The PmTry cDNA was 916 bp, which encodes 266 amino acids with a predicted molecular weight of 28.38 KDa and an isoelectric point of 4.58. Homologous analysis indicated that PmTry shared 42%similar to 91% similarity with other species. The phylogenetic tree showed that PmTry was closely related to Fenneropenaeus chinensis. Tissue expression profiles showed that PmTry was most expressed in the hepatopancreas and the lowest in the eyestalk nerve. It was expressed in the whole growth stage of P. monodon, but the relative expression level of each stage was significantly different. In addition, PmTry-524 and PmTry-798 were particularly related to growth traits of P. monodon by genotype. The SNP markers may provide a basis for genetic selection and breed improvement studies in P. monodon.

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