Genetic and transcriptomic dissection of host defense to Goss's bacterial wilt and leaf blight of maize

文献类型： 外文期刊

第一作者： Hao, Yangfan

作者： Hao, Yangfan;Hu, Ying;Lin, Jinguang;He, Cheng;Lin, Guifang;Zhao, Mingxia;Ren, Jie;Tamang, Tej Man;Liu, Sanzhen;Jaqueth, Jennifer;Li, Bailin;Park, Sunghun;Robertson, Alison E.;White, Frank F.;Fu, Junjie;Hu, Ying;Lin, Jinguang;Zhao, Mingxia;Ren, Jie

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关键词： maize disease; bacterium; Goss's wilt; disease resistance; XP-GWAS; QTL

期刊名称：G3-GENES GENOMES GENETICS （ 影响因子：2.6； 五年影响因子：3.0 ）

ISSN： 2160-1836

年卷期： 2023 年 13 卷 11 期

页码：

收录情况： SCI

摘要： Goss's wilt, caused by the Gram-positive actinobacterium Clavibacter nebraskensis, is an important bacterial disease of maize. The molecular and genetic mechanisms of resistance to the bacterium, or, in general, Gram-positive bacteria causing plant diseases, remain poorly understood. Here, we examined the genetic basis of Goss's wilt through differential gene expression, standard genome-wide association mapping (GWAS), extreme phenotype (XP) GWAS using highly resistant (R) and highly susceptible (S) lines, and quantitative trait locus (QTL) mapping using 3 bi-parental populations, identifying 11 disease association loci. Three loci were validated using near-isogenic lines or recombinant inbred lines. Our analysis indicates that Goss's wilt resistance is highly complex and major resistance genes are not commonly present. RNA sequencing of samples separately pooled from R and S lines with or without bacterial inoculation was performed, enabling identification of common and differential gene responses in R and S lines. Based on expression, in both R and S lines, the photosynthesis pathway was silenced upon infection, while stress-responsive pathways and phytohormone pathways, namely, abscisic acid, auxin, ethylene, jasmonate, and gibberellin, were markedly activated. In addition, 65 genes showed differential responses (up- or down-regulated) to infection in R and S lines. Combining genetic mapping and transcriptional data, individual candidate genes conferring Goss's wilt resistance were identified. Collectively, aspects of the genetic architecture of Goss's wilt resistance were revealed, providing foundational data for mechanistic studies.

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