TaSPL6B, a member of the Squamosa promoter binding protein-like family, regulates shoot branching and florescence in Arabidopsis thaliana

文献类型： 外文期刊

第一作者： Dong, Feiyan

作者： Dong, Feiyan;Zhang, Huadong;Li, Yaqian;Ge, Shijie;Liu, Yike;Song, Jinghan;Zhang, Jiarun;Chen, Yangfan;Dong, Feiyan;Zhang, Huadong;Zhou, Xiaoyi

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关键词： Wheat; Ectopic expression; TaSPL6B; Tillering; Light signaling pathway; Strigolactone signaling pathway

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.3； 五年影响因子：5.2 ）

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

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收录情况： SCI

摘要： Background Squamosa promoter-binding protein-like (SPL) proteins are essential to plant growth and development as plant-specific transcription factors. However, the functions of SPL proteins in wheat need to be further explored. Results We cloned and characterized TaSPL6B of wheat in this study. Analysis of physicochemical properties revealed that it contained 961 amino acids and had a molecular weight of 105 kDa. Full-length TaSPL6B transcription activity was not validated in yeast and subcellular localization analysis revealed that TaSPL6B was distributed in the nucleus. Ectopic expression of TaSPL6B in Arabidopsis led to increasing number of branches and early flowering. TaSPL6B was highly transcribed in internodes of transgenic Arabidopsis. The expression of AtSMXL6/AtSMXL7/AtSMXL8 (homologous genes of TaD53) was markedly increased, whereas the expression of AtSPL2 (homologous genes of TaSPL3) and AtBRC1 (homologous genes of TaTB1) was markedly reduced in the internodes of transgenic Arabidopsis. Besides, TaSPL6B, TaSPL3 and TaD53 interacted with one another, as demonstrated by yeast two-hybrid and bimolecular fluorescence complementation assays. Therefore, we speculated that TaSPL6B brought together TaD53 and TaSPL3 and enhanced the inhibition effect of TaD53 on TaSPL3 through integrating light and strigolactone signaling pathways, followed by suppression of TaTB1, a key repressor of tillering. Conclusions As a whole, our findings contribute to a better understanding of how SPL genes work in wheat and will be useful for further research into how TaSPL6B affects yield-related traits in wheat.

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