Influence of hapten density on immunogenicity for anti-ciprofloxacin antibody production in mice

文献类型： 外文期刊

第一作者： Hu, Kun

作者： Hu, Kun;Jiang, Yousheng;Qiu, Junqiang;Fang, Wei;Yang, Xianle;Huang, Xuanyun

作者机构：

关键词： Hapten density;conjugation;immunogenicity;ciprofloxacin

期刊名称：BIOSCIENCE TRENDS （ 影响因子：2.4； 五年影响因子：2.582 ）

ISSN： 1881-7815

年卷期： 2012 年 6 卷 2 期

页码：

收录情况： SCI

摘要： To generate antibodies against small molecules, it is necessary to couple them as haptens to large carriers such as proteins. However, the immunogenicity of the conjugates usually has no linear correlation with the hapten-protein ratio, which may lead to large variations in the character of the desired antibodies. In the present study, ciprofloxacin (CPFX) was coupled to bovine serum albumin (BSA) in five different proportions using a modified carbodiimide method. The conjugates were characterized qualitatively by spectrophotometric absorption and electrophoresis methods. Mass spectrometry and the trinitrobenzene sulfonic acid method were adopted to assay the density of conjugates quantitatively. As a result, CPFX-BSA conjugates with various hapten densities (21-30 molecules per carrier protein) were obtained. After immunization in mice, ELISA tests showed that the antisera titer increased gradually with the increase of hapten density. The antibody obtained from the mice showed high sensitivity toward CPFX. These results revealed the relationship between hapten density and immunogenicity as well as an optimized conjugation approach for immunization purposes.

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