Identification of regulatory pathways of miRna and phasiRna related to flax lignan synthesis

文献类型： 外文期刊

第一作者： Xie, Dongwei

作者： Xie, Dongwei;He, Ruihua;Ren, Xianghua;Huo, Hang;Sun, Jian;Yang, Xue;Yuan, Hongmei;Wu, Guangwen;Su, Jianguang;Sun, Jian

作者机构：

关键词： Flax; Lignan; phasiRNA; miRNA; regulatory; pathway

期刊名称：JOURNAL OF NATURAL FIBERS （ 影响因子：3.5； 五年影响因子：3.7 ）

ISSN： 1544-0478

年卷期： 2023 年 20 卷 1 期

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收录情况： SCI

摘要： Lignan is an important functional health care substance. At present, research on the molecular mechanism of flax lignan biosynthesis is primarily focused at the transcriptional level, with no research on the regulatory mechanism of small RNAs, particularly phased small interfering RNAs (phasiRNAs) related to lignan content. In this study, we used flax seeds at different developmental stages as materials to construct small RNA libraries and degradation libraries. Eighteen known and 4 new miRNA families containing 40 and 4 miRNA members, respectively, were identified. A total of 104 target genes were identified. Using small RNA data for a genome-wide search, a total of twenty-two 21-nt PHAS loci and twenty-eight 24-nt PHAS loci were obtained. Five miRNAs capable of triggering target genes to produce phasiRNAs were identified. These 5 miRNAs triggered their target genes to produce 39 phasiRNAs. Three complete miRNA-PHAS loci-phasiRNA-target gene functional regulatory pathways were identified. Furthermore, qRT-PCR confirmed an obvious regulatory relationship among the regulatory pathways. In this study, the molecular regulatory network among flax miRNAs, phasiRNAs and target genes was described from the perspective of lignan synthesis, providing an important scientific basis for the study of flax molecular breeding for high lignan content.

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