CYP6CX2 and CYP6CX3 mediate thiamethoxam resistance in field whitefly, Bemisia tabaci (Hemiptera:Aleyrodidae)

文献类型： 外文期刊

第一作者： Yang, Jing

作者： Yang, Jing;Fu, Buli;Gong, Peipan;Zhang, Chengjia;Wei, Xuegao;Yin, Cheng;Huang, Mingjiao;He, Chao;Du, Tianhua;Liang, Jinjin;Liu, Shaonan;Ji, Yao;Xue, Hu;Wang, Chao;Hu, Jinyu;Du, He;Zhang, Rong;Yang, Xin;Zhang, Youjun

作者机构：

关键词： cytochrome P450 monooxygenase; thiamethoxam; neonicotinoid; insecticide resistance

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.2； 五年影响因子：2.3 ）

ISSN： 0022-0493

年卷期： 2023 年 116 卷 4 期

页码：

收录情况： SCI

摘要： Cytochrome P450 monooxygenases (P450s) are well-known for their crucial roles in the detoxification of xenobiotics. However, whether CYP6CX2 and CYP6CX3, 2 genes from our Bemisia tabaci (B. tabaci) MED/Q genome data were associated with detoxification metabolism and confer resistance to thiamethoxam is unclear. In this study, we investigated the role of CYP6CX2 and CYP6CX3 in mediating whitefly thiamethoxam resistance. Our results showed that mRNA levels of CYP6CX2 and CYP6CX3 were up-regulated after exposure to thiamethoxam. Transcriptional levels of 2 genes were overexpressed in laboratory and field thiamethoxam resistant strains by RT-qPCR. These results indicate that the enhanced expression of CYP6CX2 and CYP6CX3 appears to confer thiamethoxam resistance in B. tabaci. Moreover, linear regression analysis showed that the expression levels of CYP6CX2 and CYP6CX3 were positively correlated with thiamethoxam resistance levels among populations. The susceptibility of whitefly adults was markedly increased after silencing 2 genes by RNA interference (RNAi) which further confirming their major role in thiamethoxam resistance. Our findings provide information to better understand the roles of P450s in resistance to neonicotinoids and suggest that these genes may be applied to develop target genes for sustainable management tactic of agricultural pests such as B. tabaci.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序