Fast Screening of 10 Kinds of Illegal Aphrodisiac Drugs in Foods by Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

文献类型： 外文期刊

第一作者： Zeng, Xi

作者： Zeng, Xi;Wang, Yu;Zhou, Heng-Xin;Zeng, Xi;Xu, Zhen-Lin;Chen, Ying-Li;Zhao, Tian-Tian

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关键词： Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; Aphrodisiac drugs; Illegal addition; alpha-Cyano-4-hydroxycinnamic acid; Sinapic acid

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.3； 五年影响因子：1.0 ）

ISSN： 0253-3820

年卷期： 2024 年 53 卷 3 期

页码：

收录情况： SCI

摘要： A matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) method was developed for simultaneous analysis of 10 kinds of aphrodisiac drugs, including sildenafil, acetildenafil, nor-acetildenafil, homosildenafil, lodenafil carbonate, sildlenafil dimer impurity, vardenafil dimer, hydroxyacetildenafil, N-desmethylsildenafil and udenafil. By using different matrices (alpha-cyano-4-hydroxycinnamic acid (HCCA) and sinapic acid (SA)), the effects of solvents, amount of matrices, laser intensity and spotting methods on the peak strength and signal intensity of MALDI-TOF MS for the aphrodisiac drugs were investigated. As a result, SA was chosen as the matrix, and then dispersed in methanol-water (50 & ratio;50, V/V), and spotted by matrix firstly and sample secondly approach with the reflect linear positive mode and laser power of 60%. Under optimal conditions, the proposed MALDI-TOF MS method could obtain stable signal, high intensity and well-repeated mass spectrometric results. The results of method validation showed a linear range from 10 to 100 ng/mL, and the regression coefficients (r) were all above 0.985. The limits of detection (LOD) were 0.1-1.0 ng/mL, and the recoveries were in the range of 72.9%-109.9% for health wine, soft capsule and instant coffee samples, while the relative standard deviations (RSDs) ranged from 0.7% to 11.3% (n = 3). The test results of 10 kinds of aphrodisiac drugs by MALDI-TOF MS were in accordance with the results of high-performance liquid chromatography-tandem mass spectrometry. This method exhibited high sensitivity, high accuracy, good anti-interference ability, low chemical solvents consumption and environmentally friendliness, and could be used to detect drugs without standards. The developed MALDI-TOF MS method could realize the non-target screening and detection of 10 kinds of illegally added aphrodisiac drugs in foods.

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