Transcription factors NtNAC028 and NtNAC080 form heterodimers to regulate jasmonic acid biosynthesis during leaf senescence in Nicotiana tabacum

文献类型： 外文期刊

第一作者： Lu, Mingyue

作者： Lu, Mingyue;Fu, Boyang;Meng, Xiao;Jia, Tiantian;Lu, Xiaoyue;Yang, Chaosha;Li, Ke;Yin, Pengcheng;Wang, Geng;Zhou, Chunjiang;Guo, Yongfeng;Li, Wei;Chi, Jina

作者机构：

关键词： Cooperation of NACs; jasmonic acid; leaf senescence; NAC transcription factors; Nicotiana tabacum; nicotine

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2024 年 75 卷 8 期

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收录情况： SCI

摘要： Plant senescence, as a highly integrated developmental stage, involves functional degeneration and nutrient redistribution. NAM/ATAF1/CUC (NAC) transcription factors orchestrate various senescence-related signals and mediate the fine-tuning underlying plant senescence. Previous data revealed that knockout of either NtNAC028 or NtNAC080 leads to delayed leaf senescence in tobacco (Nicotiana tabacum), which implies that NtNAC028 and NtNAC080 play respective roles in the regulation of leaf senescence, although they share 91.87% identity with each other. However, the mechanism underlying NtNAC028- and NtNAC080-regulated leaf senescence remains obscure. Here, we determined that NtNAC028 and NtNAC080 activate a putative jasmonic acid (JA) biosynthetic gene, NtLOX3, and enhance the JA level in vivo. We found that NtNAC028 and NtNAC080 interact with each other and themselves through their NA-terminal region. Remarkably, only the dimerization between NtNAC028 and NtNAC080 stimulated the transcriptional activation activity, but not the DNA binding activity of this heterodimer on NtLOX3. Metabolome analysis indicated that overexpression of either NtNAC028 or NtNAC080 augments both biosynthesis and degradation of nicotine in the senescent stages. Thus, we conclude that NtNAC028 cooperates with NtNAC080 and forms a heterodimer to enhance NtLOX3 expression and JA biosynthesis to trigger the onset of leaf senescence and impact secondary metabolism in tobacco. The heterodimerization of two NAC transcription factors, NtNAC028 and NtNAC080, is essential for the initiation of leaf senescence and promotes jasmonic acid biosynthesis and secondary metabolism in Nicotiana tabacum .

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