Nitrogen inhibition of nitrogenase activity involves the modulation of cytosolic invertase in soybean nodule

文献类型： 外文期刊

第一作者： Xu, Qinzhen

作者： Xu, Qinzhen;Yao, Xiaolei;Xu, Qinzhen;Yang, Wenqiang;Wang, Xin;Kuang, Huaqin;Guan, Yuefeng;Wang, Nan;Li, Suning;Qiu, Zhimin;Ke, Danxia;Ke, Danxia

作者机构：

关键词： Cytosolic invertase; GmCIN1; Nitrogen fixation; Carbon metabolism; Symbiotic nitrogen fixation; Legume

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：7.1； 五年影响因子：6.4 ）

ISSN： 1673-8527

年卷期： 2024 年 51 卷 12 期

页码：

收录情况： SCI

摘要： Legume symbiotic nitrogen fixation (SNF) is suppressed by inorganic nitrogen (N) in the soil. High N inhibition of nitrogenase activity is associated with the deprivation of carbon allocation and metabolism in nodules. However, the underlying molecular mechanisms remain unclear. Here, we identify GmCIN1, which encodes a cytosolic invertase, as a gateway for the N-tuning of sucrose utilization in nodules. GmCIN1 is enriched in mature soybean nodules, and its expression is regulated by nitrogen status. The knockout of GmCIN1 using genome editing partially mimics the inhibitory effects of N on nitrogenase activity and sugar content and the impact of high N on nodule transcriptomes. This indicates that GmCIN1 partially mediates the high N inhibition of nodule activity. Moreover, ChIP-qPCR and EMSA reveal that SNAP1/2 transcription factors directly bind to the GmCIN1 promoter. In addition, SNAP1/2 may be involved in the repression of GmCIN1 expression in mature nodules at high N concentrations. Our findings provide insights into the involvement of the transcriptional tuning of carbon (C) metabolism genes by N-signaling modulators in the N-induced inhibition of nitrogenase activity. Copyright (c) 2024, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights are reserved,

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