Rutin Attenuates the Oxidative Damage Induced by Zearalenone in Piglet Endometrial Stromal Cells via the p53 Signaling Pathway

文献类型： 外文期刊

第一作者： Wang, Chenlong

作者： Wang, Chenlong;Wang, Mengya;Yang, Yueru;Chen, Chuangjiang;Rahman, Sajid Ur;Huang, Wanyue;Wang, Xichun;Yang, Zhiping;Ding, Hongyan;Wang, Xichun

作者机构：

关键词： zearalenone; rutin; porcine endometrial stromalcells; oxidative stress; cell damage

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 50 期

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收录情况： SCI

摘要： Zearalenone (ZEA) induces oxidative damage in porcine endometrial stromal cells (ESCs), which is a critical factor affecting the growth and reproduction of female pigs. We hypothesize that rutin, a flavonoid antioxidant, can alleviate ZEA-induced cellular damage through the p53 signaling pathway. In this experiment, porcine ESCs were used as a research model. After transfection with siRNA to silence the p53 gene, rutin and ZEA were added to the cocultured porcine ESCs. Rutin notably reduced the ZEA-induced apoptosis rate, ROS, MDA, and 4-HNE levels via the p53 pathway; lowered the expression of LC3, Beclin-1, p62, Bax, and CHOP; and significantly improved cell viability, GSH-Px, T-SOD enzyme activity, and Bcl-2 expression. It also inhibited both apoptosis and autophagy. These findings suggested a novel antioxidant mechanism by which rutin mitigated the toxic effects of ZEA, highlighting the role of the p53 gene knockdown in this process.

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