Isolation and characterization of 31 polymorphic microsatellite markers in barfin flounder (Verasper moseri) and the cross-species amplification in spotted halibut (Verasper variegatus)

文献类型： 外文期刊

第一作者： Ma, Hongyu

作者： Ma, Hongyu;Chen, Songlin;Ma, Hongyu

作者机构：

关键词： Microsatellite markers;Repeat-enriched library;Barfin flounder;Verasper variegatus;Cross-species amplification

期刊名称：CONSERVATION GENETICS （ 影响因子：2.538； 五年影响因子：2.32 ）

ISSN： 1566-0621

年卷期： 2009 年 10 卷 5 期

页码：

收录情况： SCI

摘要： The construction of a genetic linkage map and evaluation of population genetic diversity both require large numbers of polymorphic molecular markers. In the present study, we report 31 polymorphic microsatellite markers, of which 28 were isolated from two repeat-enriched libraries constructed from genomic DNA, and three were detected in two genes (MCH-R1 and MCH-R2) of barfin flounder (Verasper moseri). A total of 94 alleles were detected with an average of 3.0 alleles per locus. The number of alleles, observed and expected heterozygosity per locus ranged from two to six, from 0.30 to 1.00 and from 0.33 to 0.78, respectively. Three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.0016) and no significant linkage disequilibrum between pairs of loci was found. Cross-species amplification of these markers was evaluated using the closely related species spotted halibut (Verasper variegatus). This study will potentially be useful for stock management, constructing of a genetic linkage map, mapping economically important quantitative trait loci (QTL), and studying the population genetic diversity of barfin flounder (Verasper moseri).

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