Development of high-oil maize haploid inducer with a novel phenotyping strategy

文献类型： 外文期刊

第一作者： Liu, Chenxu

作者： Liu, Chenxu;Li, Jinlong;Chen, Ming;Li, Wei;Zhong, Yu;Dong, Xin;Xu, Xiaowei;Chen, Chen;Tian, Xiaolong;Chen, Shaojiang;Dong, Xin

作者机构：

关键词： Doubled haploid; Marker-assisted selection; Kernel oil content; Haploid identification; Haploids per ear

期刊名称：CROP JOURNAL （ 影响因子：6.0； 五年影响因子：5.6 ）

ISSN： 2095-5421

年卷期： 2022 年 10 卷 2 期

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收录情况： SCI

摘要： Doubled haploid (DH) technology is important in modern maize breeding. Haploid inducers determine the efficiency of both haploid induction and identification. It has taken decades to improve the efficiency, haploid induction rate (HIR), from the similar to 2% of the ancestor haploid inducer, stock6, to the similar to 10% of modern haploid inducers. Improvement of kernel oil content (KOC) would further enhance haploid identification efficiency. Using molecular marker-assisted selection, in combine with the number of haploids per ear as phenotypic criterion, we developed a new high-oil haploid inducer line, CHOI4, with a mean HIR of 15.8% and mean KOC of 11%. High KOC of CHOI4 can achieve a mean accuracy greater than 90% in identification of haploids of different backgrounds, with reduced false discovery rates and false negative rates in comparison with the previous high-oil haploid inducer line, CHOI3. Comparison of phenotypic selection strategies suggested that the number of haploids per ear can be used as a phenotyping criterion during haploid inducer line development. CHOI4 could further increase the efficiency of large-scale DH breeding programs with lower cost. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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