Pretreated polystyrene is degraded by a microbial consortium enriched from wetland plastic waste

文献类型： 外文期刊

第一作者： Zhang, Jian

作者： Zhang, Jian;Shao, Yahui;Yang, Wenlong;Xuan, Ning;Geng, Yun;Bian, Fei;Zhang, Yingxin;Chen, Gao;Zhang, Jian;Shao, Yahui;Yang, Wenlong;Xuan, Ning;Geng, Yun;Bian, Fei;Zhang, Yingxin;Chen, Gao;Zhang, Jian;Shao, Yuanyuan;Zhang, Jian

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关键词： Constructed wetland; Microbial consortium; Polystyrene biodegradation; Pre-treatment; Degradation pathway

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：11.3； 五年影响因子：12.4 ）

ISSN： 0304-3894

年卷期： 2024 年 480 卷

页码：

收录情况： SCI

摘要： The biodegradation of polystyrene (PS), a type of plastic with aromatic rings in its polymer chain, is a critical environmental goal worldwide. Microbial degradation of PS has been reported, but the underlying mechanisms are poorly understood. Here, we constructed a microcosm wetland containing PS plastic. We isolated six highly efficient PS plastic-degrading bacterial strains and created a microbial consortium (MCs) consisting of these strains. After a 30-day incubation period, MCs-treated PS exhibited hallmarks of degradation, including -C--O- formation, reduced hydrophobicity, surface porosity, and 20 % weight loss. The efficiency of PS degradation was enhanced by using a combination of physical-chemical pretreatment and biological methods, increasing the microbial degradation rate by 20 %. Antioxidant 2246 (C23H32O2) was detected in the culture supernatant via GC-MS. Metatranscriptomic sequencing analysis provided insight into the possible metabolic pathway of PS degradation by the composite bacteria. We identified 31 highly expressed genes encoding proteins that function in carbon metabolism pathways and 34 unique proteases which catalyze the cleavage of long polymer chains. The resulting small molecules are absorbed and further degraded intracellularly by enzymes such as coenzyme synthase, hydratase, transferase, carboxylase, and dehydrogenase. These findings lay the foundation for the efficient and sustainable degradation of PS.

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