DosR Regulates the Transcription of the Arginine Biosynthesis Gene Cluster by Binding to the Regulatory Sequences in Mycobacterium bovis Bacille Calmette-Guerin

文献类型： 外文期刊

第一作者： Cui, Yingying

作者： Cui, Yingying;Dang, Guanghui;Wang, Hui;Tang, Yiyi;Lv, Mingyue;Zang, Xinxin;Cai, Zhuming;Cui, Ziyin;Cao, Jun;Liu, Siguo;Song, Ningning;Song, Ningning;Liu, Siguo;Song, Ningning

作者机构：

关键词： Mycobacterium bovis BCG; ChIP; DosR; transcriptional regulation; arginine synthesis

期刊名称：DNA AND CELL BIOLOGY （ 影响因子：3.55； 五年影响因子：3.557 ）

ISSN： 1044-5498

年卷期： 2022 年 41 卷 12 期

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收录情况： SCI

摘要： l-Arginine serves as a carbon and nitrogen source and is critical for Mycobacterium tuberculosis (Mtb) survival in the host. Generally, ArgR acts as a repressor regulating arginine biosynthesis by binding to the promoter of the argCJBDFGH gene cluster. In this study, we report that the dormancy regulator DosR is a novel arginine regulator binding to the promoter region of argC (rv1652), which regulates arginine synthesis. Phosphorylation modification promoted DosR binding to a region upstream of the promoter. Cofactors, including arginine and metal ions, had an inhibitory effect on this association. Furthermore, DosR regulatory function relies on the interaction of the 167, 181, 182, and 197 amino acid residues with an inverse complementary sequence. Arginine also binds to DosR and directly affects its DNA-binding ability. Together, the results demonstrate that DosR acts as a novel transcriptional regulator of arginine synthesis in Mycobacterium bovis bacille Calmette-Guerin.

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