Molecular characterization and functional analysis of DMRT11E in black tiger shrimp (Penaeus monodon)

文献类型： 外文期刊

第一作者： Wei, Wen-Ya

作者： Wei, Wen-Ya;Huang, Jian-Hua;Zhou, Fa-Lin;Jiang, Song;Li, Yun-Dong;Jiang, Shi-Gui;Yang, Li-Shi;Huang, Jian-Hua;Zhou, Fa-Lin;Jiang, Song;Li, Yun-Dong;Jiang, Shi-Gui;Yang, Li-Shi;Wei, Wen-Ya;Huang, Jian-Hua;Yang, Qi-Bin;Zhou, Fa-Lin;Jiang, Shi-Gui;Yang, Li-Shi;Wei, Wen-Ya;Huang, Jian-Hua;Zhou, Fa-Lin;Jiang, Song;Li, Yun-Dong;Jiang, Shi-Gui;Yang, Li-Shi

作者机构：

关键词： Penaeus monodon; DMRT11E; DM domain; Sexual differentiation; RNA interference

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.385； 五年影响因子：3.645 ）

ISSN： 2352-5134

年卷期： 2022 年 22 卷

页码：

收录情况： SCI

摘要： As a highly conserved transcription factor family, DMRTs (Doublesex and Mab-3-related transcription factors) are involved in sex determination and sexual differentiation in numerous animals. In the present study, we identified a DM (Doublesex/Mab-3)-domain gene in Penaeus monodon, which we named PmDMRT11E because it shared many similarities with DMRT11E homologs and has a close relationship in the phylogeny of different species. The three-dimensional structure and amino acid alignments indicated the stability and conservation of the DM domain. Real-time PCR analysis revealed that PmDMRT11E was highly and preferentially expressed in the testis of sub-adult shrimp. During embryogenesis, the expression level of PmDMRT11E was higher at the nauplius than at other periods. PmDMRT11E dsRNA injection significantly decreased the transcription factor Sox9 and increased ovarian marker Foxl2 expression, implying its important regulatory role in the mechanism of male determination. Moreover, dsDMRT11E caused abnormal sperm development, indicating that PmDMRT11E may be closely related to gonadal development and spermatogenesis.

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