Proline-Nitrogen Metabolic Coordination Mediates Cold Priming-Induced Freezing Tolerance in Maize

文献类型： 外文期刊

第一作者： Gai, Zhijia

作者： Gai, Zhijia;Zhang, Na;Liu, Jingqi;Cai, Lijun;Yang, Xu;Ding, Junjie;Liu, Lei;Zhang, Ao;Zhang, Yifei;Zhang, Pengfei

作者机构：

关键词： photosynthesis; proline metabolism; antioxidant enzyme; GDH/ICDH; osmoregulation; ROS

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 10 期

页码：

收录情况： SCI

摘要： Cold stress critically restricts maize seedling growth in Northeast China, yet the mechanism by which cold priming (CP) enhances cold tolerance through proline-nitrogen metabolic networks remains unclear. This study systematically investigated CP's synergistic regulation in cold-tolerant (Heyu27) and cold-sensitive (Dunyu213) maize using a two-phase temperature regime (priming induction/stress response) with physiological and multivariate analyses. CP alleviated cold-induced photosynthetic inhibition while maintaining a higher chlorophyll and photosynthetic rate, though biomass responses showed varietal specificity, with Heyu27 minimizing growth loss through optimized carbon-nitrogen allocation. Antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were pre-activated during early stress, effectively scavenging reactive oxygen species (ROS) and reducing malondialdehyde (MDA) accumulation, with Heyu27 showing superior redox homeostasis. CP enhanced proline accumulation via bidirectional enzyme regulation (upregulating triangle 1-pyrroline-5-carboxylate synthase/reductase [P5CS/P5CR], inhibiting proline dehydrogenase [ProDH]) and reprogrammed nitrogen metabolism through glutamate dehydrogenase/isocitrate dehydrogenase (GDH/ICDH)-mediated ammonium conversion to glutamate, alleviating nitrogen dysregulation while supplying proline precursors. Principal component analysis revealed divergent strategies: Heyu27 prioritized proline-antioxidant synergy, whereas Dunyu213 emphasized photosynthetic adjustments. These findings demonstrate that CP establishes "metabolic memory" through optimized proline-nitrogen coordination, synergistically enhancing osmoregulation, reactive oxygen species (ROS) scavenging, and nitrogen utilization. This study elucidates C4-specific cold adaptation mechanisms, advancing cold-resistant breeding and stress-resilient agronomy.

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