The role of gspE in regulating biofilm structure, spoilage potential, and cellular metabolism of Shewanella putrefaciens in refrigerated bigeye tuna (Thunnus obesus) in the type II secretion system

文献类型： 外文期刊

第一作者： Chu, Yuanming

作者： Chu, Yuanming;Yan, Jun;Xie, Jing;Yi, Zhengkai;Chu, Yuanming;Yan, Jun;Xie, Jing;Yan, Jun;Xie, Jing;Xie, Jing

作者机构：

关键词： Shewanella putrefaciens; Big eye tuna; Gene knockout; Metabolomics

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.9； 五年影响因子：6.1 ）

ISSN： 2212-4292

年卷期： 2025 年 71 卷

页码：

收录情况： SCI

摘要： Shewanella putrefaciens is one of the most common spoilage bacteria during the refrigerated storage of seafood. The gspE gene has been identified as a key regulator involved in the secretion of extracellular proteases in S. putrefaciens and plays a crucial role in modulating its spoilage potential. This study aimed to investigate the effects of gspE on biofilm formation, spoilage potential, and cellular metabolism in S. putrefaciens YZ08. The results demonstrated that gspE deletion altered the auto-aggregation, and biofilm formation and structure of S. putrefaciens YZ08. Furthermore, by evaluating the sensory score, TVC, TVB-N, and TMA of bigeye tuna with the strain of S. putrefaciens, it was demonstrated that the spoilage capacity of the bacteria with the gspE gene knockout was significantly weakened. Finally, metabolomic analysis indicated that gspE deletion attenuated spoilage potential by regulating amino acid synthesis and metabolism. These findings emphasize the regulatory role of the gspE gene in S. putrefaciens and suggest potential targets for future strategies for delaying seafood spoilage during refrigerated storage.

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