Haplotype variation and KASP markers for SiPSY1 - A key gene controlling yellow kernel pigmentation in foxtail millet

文献类型： 外文期刊

第一作者： Zuo, Rongjun

作者： Zuo, Rongjun;Zhang, Yanyan;Wang, Chunfang;Zhi, Hui;Zhang, Linlin;Tang, Sha;Jia, Guanqing;Diao, Xianmin;Yang, Yanbing;Guan, Yanan;Li, Shunguo;Cheng, Ruhong;Diao, Xianmin;Zuo, Rongjun;Wang, Chunfang;Shang, Zhonglin;Diao, Xianmin

作者机构：

关键词： Carotenoids; Lutein; Zeaxanthin; Marker assisted selection; Setaria italica

期刊名称：CROP JOURNAL （ 影响因子：6.6； 五年影响因子：6.5 ）

ISSN： 2095-5421

年卷期： 2023 年 11 卷 6 期

页码：

收录情况： SCI

摘要： Carotenoid biosynthesis and accumulation are important in determining nutritional and commercial value of crop products. Yellow pigmentation of mature kernels caused by carotenoids is considered a vital quality trait in foxtail millet, an ancient and widely cultivated cereal crop across the world. Genomic regions associated with yellow pigment content (YPC), lutein and zeaxanthin in foxtail millet grains were identified by genome-wide association analysis (GWAS), and SiPSY1 (Phytoene synthase 1 which regu-lates formation of the 40-carbon backbone of carotenoids) was confirmed as the main contributor to all three components by knockout and overexpression analysis. SiPSY1 was expressed in seedlings, leaves, panicles, and mature seeds, and was subcellularly localized to chloroplasts. Transcription of SiPSY1 in 15 DAP immature grains was responsible for YPC in mature seeds. Selection of SiPSY1 combined with increased YPC in mature grains during domestication of foxtail millet was confirmed. Haplotype analysis suggested that expression level of SiPSY1 could be a selection target for future breeding programs, and a KASP marker was developed for selection of favorable SiPSY1 alleles in breeding. The results of this work will benefit nutritional and commercial improvement of foxtail millet varieties, as well as other cereal crops. (c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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