Identification and characterization of a novel copper transporter gene family TaCT1 in common wheat

文献类型： 外文期刊

第一作者： Li, Haoxun

作者： Li, Haoxun;Fan, Renchun;Li, Libin;Wei, Bo;Li, Guoliang;Gu, Liqing;Wang, Xianping;Zhang, Xiangqi;Li, Haoxun;Li, Guoliang;Gu, Liqing;Li, Haoxun;Li, Libin;Li, Guoliang

作者机构：

关键词： Golgi apparatus; phylogenetic analysis; site-directed mutagenesis; virus-induced gene silencing

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：7.228； 五年影响因子：7.791 ）

ISSN： 0140-7791

年卷期： 2014 年 37 卷 7 期

页码：

收录情况： SCI

摘要： Copper is an essential micronutrient for plant growth and development, and copper transporter plays a pivotal role for keeping copper homeostasis. However, little is known about copper transporters in wheat. Here, we report a novel copper transporter gene family, TaCT1, in common wheat. Three TaCT1 homoeologous genes were isolated and assigned to group 5 chromosomes. Each of the TaCT1 genes (TaCT1-5A, -5B or -5D) possesses 12 transmembrane domains. TaCT1 genes exhibited higher transcript levels in leaf than in root, culm and spikelet. Excess copper down-regulated the transcript levels of TaCT1 and copper deficiency-induced TaCT1 expression. Subcellular experiments localized the TaCT1 to the Golgi apparatus. Yeast expression experiments and virus-induced gene silencing analysis indicated that the TaCT1 functioned in copper transport. Site-directed mutagenesis demonstrated that three amino acid residues, Met(35), Met(38) and Cys(365), are required for TaCT1 function. Phylogenetic and functional analyses suggested that homologous genes shared high similarity with TaCT1 may exist exclusively in monocot plants. Our work reveals a novel wheat gene family encoding major facilitator superfamily (MFS)-type copper transporters, and provides evidence for their functional involvement in promoting copper uptake and keeping copper homeostasis in common wheat.

分类号： Q948

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