Saccharothrix luteola sp. nov., a novel cellulose--degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix

文献类型： 外文期刊

第一作者： He, Chuan

作者： He, Chuan;Sun, Xiujun;Huang, Zhenzhen;Wang, Zishan;Luo, Xianxian;Song, Jia;Wang, Xiangjing;Zhao, Junwei;Xiang, Wensheng;Wang, Xiangjing

作者机构：

关键词： 16S rRNA gene; genome characterization; novel species; taxonomy

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）

ISSN： 1466-5026

年卷期： 2022 年 72 卷 10 期

页码：

收录情况： SCI

摘要： A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach. Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2%), Saccharothrix saharensis SA152T (99.0%), Saccharothrix texasensis DSM 44231T (98.5%) and Saccharothrix xinjiangensis NBRC 101911T (98.5%). Physiological and chemotaxonomic characteristics of the strain further supported its affiliation to the genus Saccharothrix. The whole-cell sugars contained galactose, ribose and mannose. The polar lipids contained diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant menaquinones were MK-9(H 0), MK-9(H 2), MK-9(H 4) and MK-10(H 4). The major fatty acids were iso-C 16 :0, C 16:0, anteiso-C 17:0, iso-C 15:0 and iso-C 17 :0. The genomic DNA G+C content was 71.8mol%. The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T were 40.1% (37.6-42.6%), 38.soap8% (36.3-41.3%) and 44.8 % (42.2-47.3 %) and the ANI values between them were determined to be 90.2, 89.8 and 91.7%, the results indicated that strain NEAU-S10T could be distinguished from its reference strains. The assembled genome sequence of strain NEAU-S10T was found to be 10305 394 bp long. The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) revealed 8 994 protein-coding genes. Genomic analysis and Congo red staining test indicated that strain NEAU-S10T had the potential to degrade cellulose. The genomic and phenotypic results indicate that strain NEAU-S10T represents a novel species of the genus Saccharothrix, for which the name Saccharothrix luteola sp. nov. is proposed, with NEAU-S10T (=CCTCC AA 2020037T=JCM 34800T) as the type strain.

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