Lowering energy consumption for fermentable sugar production from Ramulus mori: Engineered xylanase synergy and improved pretreatment strategy

文献类型： 外文期刊

第一作者： You, Shuai

作者： You, Shuai;Zhang, Yi-Xin;Shi, Fan;Zhang, Wen-Xin;Zhang, Sheng;Zhao, Wei-Guo;Wang, Jun;You, Shuai;Zhao, Wei-Guo;Wang, Jun;Li, Jing;Chen, Zhong-Li

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关键词： Laboratory evolution; Ramulus mori; Seawater pretreatment; Synergistic enzymatic hydrolysis; Low energy consumption

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.889； 五年影响因子：11.139 ）

ISSN： 0960-8524

年卷期： 2022 年 344 卷

页码：

收录情况： SCI

摘要： Biorefinery of Ramulus mori with lower energy consumption through improved enzyme and pretreatment strategies was reported. Directed evolution and saturation mutagenesis were used for the modification of xylanase, the yield of fermentable sugars and the degree of synergy (DS) were determined for different pretreatment (seawater/non-seawater) and enzyme treatment groups (xylanase/cellulase/co-treatment). The dominant mutant I133A/Q143Y of Bispora sp. xylanase XYL10C_Delta N was obtained with improved specific activity (1860 U/ mg), catalytic efficiency (1150 mL/s center dot mg) at 40 degrees C, and thermostability (T50 increased by 7 degrees C). With the pretreatment of seawater immersion, the highest yield of fermentable sugars for Ramulus mori at 40 degrees C reached 199 mu mol/g when hydrolyzed with cellulase and I133A/Q143Y, with the highest DS of 2.6; this was 4.5-fold that of the group hydrolyzed by cellulase alone with non-seawater pretreatment. Thus, bioconversion of reducing sugar from Ramulus mori was improved significantly at lower temperatures, which provides an efficient and energy -saving way for biofuel production.

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