Integrative and conjugative elements in streptococci can act as vectors for plasmids and translocatable units integrated via IS 1216E

文献类型： 外文期刊

第一作者： Yang, Qin

作者： Yang, Qin;Zhu, Yao;Wang, Lingli;Liu, Wenyu;Yang, Wenlin;Liu, Siguo;Zhang, Wanjiang;Schwarz, Stefan;Schwarz, Stefan;Yang, Qin

作者机构：

关键词： IS 1216E; Integrative and conjugative elements; Translocatable units; Plasmid; Streptococci

期刊名称：INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS （ 影响因子：10.8； 五年影响因子：7.2 ）

ISSN： 0924-8579

年卷期： 2023 年 61 卷 5 期

页码：

收录情况： SCI

摘要： Mobile genetic elements (MGEs), such as integrative and conjugative elements (ICEs), plasmids and translocatable units (TUs), are important drivers for the spread of antibiotic resistance. Although ICEs have been reported to support the spread of plasmids among different bacteria, their role in mobilizing resistance plasmids and TUs has not yet been fully explored. In this study, a novel TU bearing optrA, a novel non-conjugative plasmid p5303-cfrD carrying cfr(D) and a new member of the ICESa2603 fam-ily, ICESg5301 were identified in streptococci. Polymerase chain reaction (PCR) assays revealed that three different types of cointegrates can be formed by IS1216E-mediated cointegration between the three differ-ent MGEs, including ICESg5301::p5303-cfrD::TU, ICESg5301::p5303-cfrD, and ICESg5301::TU. Conjugation assays showed that ICEs carrying p5303-cfrD and/or TU successfully transferred into recipient strains, thereby confirming that ICEs can serve as vectors for other non-conjugative MGEs, such as TUs and p5303-cfrD. As neither the TU nor plasmid p5303-cfrD can spread on their own between different bacte-ria, their integration into an ICE via IS1216E-mediated cointegrate formation not only increases the plas-ticity of ICEs, but also furthers the dissemination of plasmids and TUs carrying oxazolidinone resistance genes.(c) 2023 Elsevier Ltd and International Society of Antimicrobial Chemotherapy. All rights reserved.

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