Inefficiency of ribavirin to eliminate apple scar skin viroid from apple plants

文献类型： 外文期刊

第一作者： Hu, Guojun

作者： Hu, Guojun;Dong, Yafeng;Zhang, Zunping;Fan, Xudong;Ren, Fang

作者机构：

关键词： Apple scar skin viroid; Ribavirin; Hydroponic culture; In vitro culture; Virus elimination

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.726； 五年影响因子：2.859 ）

ISSN： 0167-6857

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收录情况： SCI

摘要： Apple scar skin viroid (ASSVd) is a major pathogen of apples that can result in significant economic losses in the apple industry. In this study, ASSVd- and apple stem grooving virus (ASGV)-infected Maluspumila 'Spy 227' apple plants were treated with ribavirin to determine its elimination efficiency. Ribavirin at 25 and 50 mu g/mL (R25 and R50) did not inhibit ASSVd in apple plants regenerated from hydroponic culture. However, the elimination rates of ASGV in R25- and R50-treated plants were 50.0% and 53.3%, respectively. The ability of different concentrations of ribavirin (10, 20, and 30 mu g/mL, R10, R20, and R30) to eliminate ASSVd and ASGV from in vitro plants was also evaluated. No phytotoxicity was observed during the treatment. Ribavirin increased the growth and proliferation of in vitro apple plants. The titers of ASSVd were similar throughout the treatment period, but ASGV declined. The concentration and duration of ribavirin also affected the regeneration of in vitro plants. The average survival rate of plants (80.8%) regenerated from R30 was similar to that of CK (82.5%) and was 14.2% and 16.3% higher than that of R10 and R20, respectively. Quantitative real-time PCR was used to assess the eradication efficiency of regenerated plants. The average elimination rates of ASSVd and ASGV were 1.6% and 78.7%, respectively. A low elimination rate of ASSVd was found in R30 (2.8%) at 45 days, and it was still less than 10% at the end of the treatment. Key message This study found that it was difficult for ribavirin to eliminate apple scar skin viroid from apple plants.

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