A casein kinase TaCK2α interacts with TaCK2β and contributes to wheat resistance to Puccinia triticina

文献类型： 外文期刊

第一作者： Feng, Yan

作者： Feng, Yan;Zhang, Yanjun;Ma, Qiuying;Cui, Zhongchi;Liu, Daqun;Wang, Haiyan;Zhang, Yanjun;Wang, Shen

作者机构：

关键词： Casein kinase 2; Puccinia triticina; Wheat; Protein interaction; Pattern-triggered immunity

期刊名称：PHYTOPATHOLOGY RESEARCH （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN： 2096-5362

年卷期： 2025 年 7 卷 1 期

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收录情况： SCI

摘要： Casein kinase 2 (CK2), a key multifunctional protein kinase in plant cells, is ubiquitously expressed and plays a crucial role in survival under various stress conditions. However, the role of CK2 in the interaction between wheat and Puccinia triticina (Pt) remains ambiguous. In our previous study, a CK2 gene, known as TaCK2 alpha, was identified in the near-isogenic wheat line TcLr19 inoculated with Pt through RNA-Sequence analysis. In the current study, quantitative real-time polymerase chain reaction (qPCR) analysis revealed that the expression of TaCK2 alpha was upregulated by the Pt race THTS and signaling molecules, indicating its potential involvement in the wheat-Pt interaction, particularly in relation to salicylic acid (SA) signaling. Antifungal activity assays and virus-induced gene silencing (VIGS) analysis further validated the role of TaCK2 alpha in regulating wheat resistance to Pt. Using a combination of pulldown assays and mass spectrometry (MS), 24 potential interacting targets of TaCK2 alpha were identified in wheat. Among these targets, the interaction between TaCK2 alpha and TaCK2 beta was confirmed through Yeast two-hybrid (Y2H) and Co-Immunoprecipitation (Co-IP). Additionally, the subcellular localization of TaCK2 alpha was found to be altered by CK2 beta. TaCK2 alpha plays a positive role in reactive oxygen species (ROS) induction and callose deposition in wheat, whereas TaCK2 beta contributes to the pattern-triggered immunity (PTI) response by interacting with TaCK2 alpha. Collectively, our results demonstrate that TaCK2 alpha targets TaCK2 beta to mediate wheat resistance against Pt through PTI, providing a solid basis for further investigation into the molecular mechanisms underlying CK2-dependent wheat resistance to biotic stress.

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