Viromics-based precision diagnosis of reproductive abnormalities in cows reveals a reassortant Akabane disease virus

文献类型： 外文期刊

第一作者： Sun, Yue

作者： Sun, Yue;Wang, Huiyu;Sun, Zheng;Yi, Le;Tu, Changchun;He, Biao;Zhang, Ru;Yang, Yanling;Tu, Changchun

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关键词： Virome; Akabane disease virus; Arbovirus; Cattle; Reassortment

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.6； 五年影响因子：2.7 ）

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年卷期： 2024 年 20 卷 1 期

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收录情况： SCI

摘要： BackgroundAt the end of 2021, an epidemic of reproductive abnormalities in cows occurred in Jilin Province, China, posing an urgent need for a rapid diagnosis.ResultsTo identify the cause of the disease, a total of 172 samples were collected from 21 dead calves and 45 aborting or pregnant cows in 10 farms across the province. Routine PCR or RT-PCR detection did not find any common abortion-related agents. We then employed the viromics-based precision diagnosis method to analyze these samples, and the read-based annotation showed signals of an Akabane disease virus (AKAV) in some libraries. To further identify the virus, nested RT-PCR detection revealed that 52.3% (11/21) of dead calves and 26.6% (12/45) of cows were positive for the virus. Phylogenetic analysis of the partial fragments showed that the S segment of the virus was 100% identical to the Chinese strain TJ2016, but its M and L segments shared 94.3% and 96.5% identities with an Israeli strain.ConclusionsThe viromic and molecular results suggested that these animals were infected with a reassortant AKAV. Coupled with the clinical signs, the virus should be responsible for the epizootic, highlighting that molecular and serological surveys of the virus in cows during early pregnancy, as well as ecological investigation in its arthropod vectors, are necessary.

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