Involvement of CgHSFB1 in the regulation of self-incompatibility in 'Shatian' pummelo

文献类型： 外文期刊

第一作者： Liu, Chenchen

作者： Liu, Chenchen;Zheng, Xin;Hu, Jianbing;Xu, Qiang;Wen, Hao;Zhang, Zhezhong;Liu, Ran;Xie, Zongzhou;Ye, Junli;Deng, Xiuxin;Chai, Lijun;Deng, Xiuxin;Chai, Lijun;Chen, Xiangling

作者机构：

关键词： Self-incompatibility; Citrus; RNA-Seq; S-RNase; CgHSFB1

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：3.9； 五年影响因子：4.6 ）

ISSN： 0167-4412

年卷期： 2024 年 114 卷 4 期

页码：

收录情况： SCI

摘要： As self-incompatibility is a major issue in pummelo breeding and production, its mechanism in citrus was analyzed to improve breeding efficiency and reduce production costs. Rutaceae belongs to S-RNase type of gametophytic self-incompatibility. While the function of S-RNase/SLF and the mechanism of self-incompatibility have been studied extensively, the transcriptional regulation of S-RNase has been less studied. We performed transcriptome sequencing with the styles of 'Shatian' pummelo on the day of anthesis and 1-5 days before anthesis, and found that the transcript level of S-RNase gradually decreased with flower development. By analyzing differentially expressed genes and correlation with the expression trend of S-RNase, we identified a candidate gene, CgHSFB1, and utilized biochemical experiments such as yeast one-hybrid assay, electrophoretic mobility shift assay and dual-luciferase assay, as well as transient transformation of citrus calli and Citrus microcarpa and demonstrated that CgHSFB1 could directly bind to the S-1-RNase promoter and repress the expression of S-1-RNase, which is involved in the pummelo self-incompatibility response. In contrast, CgHSFB1 did not bind to the promoter of S-2-RNase, and there was specificity in the regulation of S-RNase. Key message Transcription factor CgHSFB1 regulates the expression of S -RNase and is thus involved in self-incompatibility in citrus.

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