Colorimetric Sensing of Cysteine in serum by the competitive adsorption toward AuNPs between Cysteine and ATP

文献类型： 外文期刊

第一作者： Zhao, Lingzhi

作者： Zhao, Lingzhi;Miao, Yanqing;Liu, Chunye;Zhao, Liu;Zhao, Lingzhi

作者机构：

关键词： colorimetric;cysteine;reduced glutathione;homocysteine;Gold nanoparticles (AuNPs)

期刊名称：PROCEEDINGS OF THE 3RD INTERNATIONAL CONFERENCE ON MATERIAL, MECHANICAL AND MANUFACTURING ENGINEERING

ISSN： 2352-5401

年卷期： 2015 年 27 卷

页码：

收录情况： SCI

摘要： Based on the dependence between the AuNPs size and the naked-eye visible color change. This study was designed to develop a highly selective and sensitive towards colorimetric sensing of cysteine in fetal bovine serum by the competitive binding of ATP and thiol compounds (cysteine and homocysteine) towards gold nanoparticles (AuNPs). The as-established method has high sensitivity and selectivity, the linearity range was 3 mu M-30 mu M with the quantification limit of 1 mu M. The method demonstrated here can be applied to detect cysteine in fetal bovine serum without interference from other amino acids and bovine serum albumin (BSA), which is envisaged to be applicable to understanding the chemical essence involved in physiological and pathological events associated with cysteine.

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