Cloning and characterization of the pyrG gene of Pleurotus ostreatus and Pleurotus eryngii
文献类型: 外文期刊
第一作者: Yin, Yonggang
作者: Yin, Yonggang;Li, Ming;Yin, Yonggang;Liu, Yu;Wang, Shouxian;Niu, Liying;Zhao, Shuang;Geng, Xiaoli;Xu, Feng;Li, Huamin
作者机构:
关键词: Pleurotus ostreatus;Pleurotus eryngii;pyrG;nested PCR;chromosome walking
期刊名称:AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH ( 影响因子:0.539; 五年影响因子:0.564 )
ISSN: 1996-0808
年卷期: 2012 年 6 卷 12 期
页码:
收录情况: SCI
摘要: Pleurotus spp. are well known and important cultivated mushrooms. However, the pyrG gene in Pleurotus spp., which is used as a bio-safe selective marker in transformation systems, has not yet been characterized. In the present study, nested degenerated PCR was used to clone conserved fragments of the pyrG gene from Pleurotus ostreatus and Pleurotus eryngii. Chromosome walking technology was then used to obtain the full-length DNA sequence of pyrG. Finally, reverse transcriptase (RT) PCR was used to obtain the pyrG cDNA sequence. The cDNA sequence of P. ostreatus and P. eryngii were all 813 bp in length and encoded 246 amino acids. Comparison of the DNA sequences with cDNA sequences of both P. ostreatus and P. eryngii indicated that pyrG genes of these two strains consisted of two introns and their deduced amino acid sequence showed 93.09% similarity. Moreover, the 5'-flanking region of these two genes was analyzed.
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