Development of molecular markers linked to the traits of non-acid/acid fruit of peach

文献类型： 外文期刊

第一作者： Wu, Jun

作者： Wu, Jun;Shu, Huairui;Zhang, Kaichun;Jiang, Lijie

作者机构：

关键词： Prunus persica (L.) batsch;AFLP;SCAR

期刊名称：PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON STRUCTURAL AND FUNCTIONAL GENOMICS OF HORTICULTURAL PLANTS

ISSN： 0567-7572

年卷期： 2007 年 763 期

页码：

收录情况： SCI

摘要： AFLP and bulked segregant analysis were used to screen markers linked to the non-acid/acid trait of peach fruit controlled by D/d gene. Of 64 pairs of primer combinations, twelve amplified polymorphic DNA fragments between non-acid bulk and acid bulk. Only two specific fragments were linked to acid trait (recessive d location), after investigating the progenies. Specific fragments TA-CTC140 and AT-CTA(200) were derived from primers E-TA/M-CTC and E-AT/M-CTA. The linkage distances to D/d gene were 1.47cM and 2.99cM, respectively. Specific primers BFPA(1)/A(2) and BFPB1/B-2 were designed according to the sequences of two specific fragments. With primers BFPB1/B-2, genomic DNA amplified the polymorphic band AT-CTA(158) in acid bulks and individuals. The AFLP marker AT-CTA200 was converted into a SCAR marker. The linkage distance of marker AT-CTA158 to d gene was the same as that of the original AFLP marker. However, the AFLP marker TA-CTC140 was not converted into a SCAR marker. Polymorphism disappeared in both parents and progenies when amplified 'With primer pair A(1)/A2. http://www.actahort.org/members/showpdf?booknrarnr=763_17

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