Cloning and characterization of Agp1, the gene encoding the small subunit of ADP-glucose pyrophosphorylase from wheat and its relatives

文献类型： 外文期刊

第一作者： Zhang, Ling-Ling

作者： Zhang, Ling-Ling;Zhong, Xiao-Juan;Jiang, Qian-Tao;Ma, Jian;Qi, Peng-Fei;Li, Wei;Chen, Guo-Yue;Lan, Xiu-Jin;Deng, Mei;Wei, Yu-Ming;Zheng, You-Liang;Wang, Qing;Lu, Zhen-Xiang;Pu, Zong-Jun

作者机构：

关键词： ADP-glucose pyrophosphorylase small subunit;cDNA sequence;functional analysis;gene structure;glycogen synthesis

期刊名称：BIOLOGIA （ 影响因子：1.35； 五年影响因子：1.139 ）

ISSN： 0006-3088

年卷期： 2017 年 72 卷 12 期

页码：

收录情况： SCI

摘要： ADP-glucose pyrophosphorylase (AGP) consists of two large (AGP-L) and two small (AGP-S) subunits; it regulates the limiting step in the biosynthesis of starch. Here, we isolated the full-length cDNA sequence of the AGP-S gene (designed as Agp1) from wheat and whole genomic sequences from relatives of wheat. The coding region of Agp1 ranged from 5948 bp to 7666 bp and contained 9 exons and 8 introns. The full-length cDNA sequence of Agp1 encoded a polypeptide containing 473 amino acids without transit peptides. The sequence alignment and phylogenetic tree analyses indicated that the Agp1 cDNA sequence was highly conserved (99.7%) across the diploid Triticeae species examined in this study. Variations in introns caused the Agp1 genomic sequences from wheat relatives to be less similar (79.5%), and Agp1 sequences from the B genome donor species were divergent. In developing grains, rapid increase in Agp1 expression at prophase was closely followed by the accumulation of starch, and a significant (P < 0.001) correlation was observed between them. The heterogeneous mixtures of small and large subunits from different wheat species restarted glycogen synthesis in the Escherichia coli AC70R1-504 cells, which are deficient in endogenous AGP activity (glg C-/-). Our data provide useful information to evaluate and utilize Agp1 sequences in Triticeae species.

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