A Genome-Wide Expression Profile of Salt-Responsive Genes in the Apple Rootstock Malus zumi

文献类型： 外文期刊

第一作者： Liu, Jia

作者： Liu, Jia;Jiang, Yuzhuang;Xu, Xuefeng;Han, Zhenhai;Kong, Jin;Liu, Jia;Tan, Dunxian;Allan, Andrew C.

作者机构：

关键词： salt stress;Malus zumi;microarray;salt-responsive gene;interaction network

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2013 年 14 卷 10 期

页码：

收录情况： SCI

摘要： In some areas of cultivation, a lack of salt tolerance severely affects plant productivity. Apple, Malus x domestica Borkh., is sensitive to salt, and, as a perennial woody plant the mechanism of salt stress adaption will be different from that of annual herbal model plants, such as Arabidopsis. Malus zumi is a salt tolerant apple rootstock, which survives high salinity (up to 0.6% NaCl). To examine the mechanism underlying this tolerance, a genome-wide expression analysis was performed, using a cDNA library constructed from salt-treated seedlings of Malus zumi. A total of 15,000 cDNA clones were selected for microarray analysis. In total a group of 576 cDNAs, of which expression changed more than four-fold, were sequenced and 18 genes were selected to verify their expression pattern under salt stress by semi-quantitative RT-PCR. Our genome-wide expression analysis resulted in the isolation of 50 novel Malus genes and the elucidation of a new apple-specific mechanism of salt tolerance, including the stabilization of photosynthesis under stress, involvement of phenolic compounds, and sorbitol in ROS scavenging and osmoprotection. The promoter regions of 111 genes were analyzed by PlantCARE, suggesting an intensive cross-talking of abiotic stress in Malus zumi. An interaction network of salt responsive genes was constructed and molecular regulatory pathways of apple were deduced. Our research will contribute to gene function analysis and further the understanding of salt-tolerance mechanisms in fruit trees.

分类号：

  • 相关文献

[1]Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of peanut (Arachis hypogaea L.). Chen, Na,Chi, Xiaoyuan,Zhang, Zhimeng,Pan, Lijuan,Chen, Mingna,Wang, Tong,Wang, Mian,Yang, Zhen,Yu, Shanlin,Su, Maowen,Chi, Xiaoyuan.

[2]Comprehensive Proteomic Analysis of Lysine Acetylation in the Foodborne Pathogen Trichinella spiralis. Yang, Yong,Bai, Xue,Liu, Xiaolei,Liu, Mingyuan,Yang, Yong,Zhang, Peihao,Cai, Wei,Tong, Mingwei,Cai, Xuepeng,Cai, Xuepeng,Luo, Xuenong,Vallee, Isabelle,Zhou, Yonghua. 2018

[3]Genome-wide identification, classification, and analysis of heat shock transcription factor family in Chinese cabbage (Brassica rapa pekinensis). Huang, X. Y.,Tao, P.,Li, B. Y.,Wang, W. H.,Yue, Z. C.,Lei, J. L.,Zhong, X. M.,Huang, X. Y.. 2015

[4]Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular mycorrhizae under NaCl stress. ZhongQun, He,ChaoXing, He,ZhiBin, Zhang,ZhiRong, Zou,HuaiSong, Wang.

[5]A comparative transcriptome analysis of two sets of backcross inbred lines differing in lint-yield derived from a Gossypium hirsutum x Gossypium barbadense population. Wu Man,Liyuan Zhang,Xihua Li,Xiaobing Xie,Wenfeng Pei,Jiwen Yu,Shuxun Yu,Jinfa Zhang. 2016

[6]Difference in miRNA expression profiles between two cotton cultivars with distinct salt sensitivity. Zujun Yin,Yan Li,Jiwen Yu,Yudong Liu,Chunhe Li,Xiulan Han,Fafu Shen.

[7]Microarray Analysis on the Differences of Gene Expression in Longissimus Dorsi Muscle Tissue Between 1 and 24 Months Chinese Red Steppes. Qin, Lihong,Zhang, Jiabao,Zhao, Zhihui,Zhang, Guoliang,Cao, Yang,Zhao, Yumin. 2011

[8]Microarray analysis of differentially expressed genes engaged in fruit development between Prunus mume and Prunus armeniaca. Li, Xiaoying,Korir, Nicholas Kibet,Shangguan, Lingfei,Han, Jian,Fang, Jinggui,Wang, Yuzhu,Liu, Lili,Chen, Ming. 2012

[9]Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives. Wu, Tao,Yang, Chunyan,Ding, Baoxu,Feng, Zhiming,Wang, Qian,He, Jun,Jiang, Ling,Wan, Jianmin,Wan, Jianmin,Tong, Jianhua,Xiao, Langtao. 2016

[10]Biological control of bacterial wilt in Arabidopsis thaliana involves abscissic acid signalling. Feng, Dong Xin,Tasset, Celine,Hanemian, Mathieu,Barlet, Xavier,Tremousaygue, Dominique,Deslandes, Laurent,Marco, Yves,Feng, Dong Xin,Tasset, Celine,Hanemian, Mathieu,Barlet, Xavier,Tremousaygue, Dominique,Deslandes, Laurent,Marco, Yves,Feng, Dong Xin,Hu, Jian. 2012

[11]Advance in genetical genomics. Liu Xiu-Yan,Xie Zheng-Miao,Chen Hui-Zhe. 2006

[12]Transcriptome identification of the resistance-associated genes (RAGs) to Aspergillus flavus infection in pre-harvested peanut (Arachis hypogaea). Chen, Xiao-Ping,Li, Hai-Fen,Liu, Hai-Yan,Hong, Yan-Bin,Liang, Xuan-Qiang,Wang, Tong,Li, Ling,Liang, Xuan-Qiang,Wang, Tong,Yang, Qing-Li,Chi, Xiao-Yuan,Yang, Zhen,Yu, Shan-Lin. 2013

[13]Recent Advances in Mycotoxin Determination for Food Monitoring via Microchip. Man, Yan,Liang, Gang,Li, An,Pan, Ligang,Man, Yan,Liang, Gang,Li, An,Pan, Ligang,Man, Yan,Liang, Gang,Li, An,Pan, Ligang. 2017

[14]Genome Array on Differentially Expressed Genes of Skin Tissue in Cashmere Goat at Early Anagen of Cashmere Growth Cycle Using DNA Microarray. Di Jiang,Xu Xin-ming,Ainiwaer, Lazate,Zhang Yan-hua,Tian Ke-chuan,Yu Li-juan,Wu Wei-wei,Tulafu, Hanikezi,Fu Xue-feng,Yasen, Marzeya. 2014

[15]Gene Expression Profiling and Identification of Resistance Genes to Aspergillus flavus Infection in Peanut through EST and Microarray Strategies. Bhatnagar, Deepak,Yu, Jiujiang,Guo, Baozhu,Fedorova, Natalie D.,Wan, Chun-Hua,Wang, Wei,Nierman, William C.,Chen, Xiaoping,Chen, Xiaoping,Nierman, William C.. 2011

[16]Transcriptional profiles in bursal B-lymphoid DT40 cells infected with very virulent infectious bursal disease virus. Quan, Rong,Zhu, Shanshan,Wei, Li,Wang, Jing,Yan, Xu,Li, Zixuan,Liu, Jue. 2017

[17]Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray. Bonnema, Guusje,Kang, Jungen,Zhang, Guoyu,Fang, Zhiyuan,Wang, Xiaowu.

[18]Identification of tapetum-specific genes by comparing global gene expression of four different male sterile lines in Brassica oleracea. Ma, Yuan,Zhu, Yingguo,Ma, Yuan,Wu, Jian,Wang, Xiaowu,Kang, Jungen.

[19]Data Mining in Networks of Differentially Expressed Genes during Sow Pregnancy. Wang, Ligang,Zhang, Longchao,Li, Yong,Li, Wen,Luo, Weizhen,Cheng, Duxue,Yan, Hua,Ma, Xiaojun,Liu, Xin,Liang, Jing,Zhao, Kebin,Wang, Lixian,Song, Xin.

[20]Dynamic expression analysis of early response genes induced by potato virus Y in PVY-resistant Nicotiana tabacum. Chen, Shuai,Li, Fengxia,Liu, Dan,Jiang, Caihong,Cui, Lijie,Shen, Lili,Liu, Guanshan,Yang, Aiguo.

作者其他论文 更多>>

微信小程序