First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha

文献类型: 外文期刊

第一作者: Ma, Duanzheng

作者: Ma, Duanzheng;Wang, Hexiang;Sekete, Malota;Ma, Duanzheng;Wang, Hexiang;Wang, Bo;Ng, Tzibun

作者机构:

关键词: Ribonuclease;Mushroom;Purification

期刊名称:SPRINGERPLUS ( 影响因子:1.13; 五年影响因子:1.29 )

ISSN: 2193-1801

年卷期: 2012 年 1 卷

页码:

收录情况: SCI

摘要: A 45-kDa ribonuclease (RNase) was purified from dried fruiting bodies of the wild mushroom Amanita hemibapha. It was adsorbed on DEAE-cellulose, S-sepharose, and finally purified on Superdex 75. The RNase exhibited maximal RNase activity at pH 5 and in a temperature range between 60-70 degrees C. It demonstrated no ribonucleolytic activity toward four polyhomoribonucleotides. The amino acid sequence analysis (GDDETFWEHEWAK) showed this RNase was a ribonuclease T2-like RNase. It exhibited strong inhibitory activity against HIV-1 reverse transcriptase (HIV-1 RT) with an IC50 of 17 mu M.

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