Molecular epidemiology of colistin-resistant Enterobacteriaceae in inpatient and avian isolates from China: high prevalence of mcr-negative Klebsiella pneumoniae

文献类型： 外文期刊

第一作者： Wang, Xiaojuan

作者： Wang, Xiaojuan;Wang, Ruobing;Jin, Longyang;Zhang, Yawei;Wang, Qi;Chen, Hongbin;Wang, Hui;Liu, Yuqing;Zhao, Min;Qi, Xiaomei;Qi, Xiaomei;Qi, Xiaomei

作者机构：

关键词： Klebsiella pneumoniae;Escherichia coli;Colistin;MCR-1

期刊名称：INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS （ 影响因子：5.283； 五年影响因子：5.209 ）

ISSN： 0924-8579

年卷期： 2017 年 50 卷 4 期

页码：

收录情况： SCI

摘要： Whether chromosomal and transmissible mechanisms contribute simultaneously to colistin resistance in Klebsiella pneumoniae and Escherichia coli remains unknown. This study aims to identify the underlying mechanisms of colistin resistance in inpatient and avian K. pneumoniae and E. coli in China. We retrospectively screened 2353 Enterobacteriaceae isolates from inpatients at multiple centers during 20112014, and 168 avian isolates from one slaughterhouse in 2013 for the presence of MCR-1/MCR-2. Mutations and transcriptional levels of the chromosomal RamA, PhoPQ, and PmrAB genes were determined by PCR and RT-qPCR. The transferability and genetic characteristics of the underlying colistin-resistance genes were detected by conjugation and whole-genome sequencing. The MIC90 for colistin in colistin-resistant K. pneumoniae (ColRKP, 128 mg/L, N = 17) was 16-fold higher than in colistin-resistant E. coli (ColREC, 8mg/L, N = 33). The dominant sequence types of ColRKP were ST2018 and ST37, whereas ColREC displayed diversity. The chromosomal genes ramA, pmrB, and phoQ were not associated with colistin resistance in ColRKP. The transcriptional levels of PmrB in ColREC were 7.5-fold greater than in colistin-susceptible isolates. The carrying rates of MCR-1 in ColREC and ColRKP were 100% (33/33) and 23.5% (4/17), respectively. Plasmid IncI2 (similar to 60 kb) carrying MCR-1 could be transferred to recipient E. coli EC600 with frequencies ranging from 8.74 x 10(-6) to 1.31 x 10(-4). No transferable genes were identified in mcr-1-negative ColRKP. MCR-1 combined with upregulated PmrB was associated with low-level colistin resistance in ColREC. However, two-thirds of the ColRKP isolates were mcr-negative and need to be studied further. (C) 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

分类号：

  • 相关文献

[1]Fitness Advantage of mcr-1-Bearing IncI2 and IncX4 Plasmids in Vitro. Wu, Renjie,Yi, Ling-xian,Yu, Lin-feng,Wang, Jing,Liu, Yiyun,Chen, Xiaojie,Lv, Luchao,Yang, Jun,Liu, Jian-Hua,Chen, Xiaojie. 2018

[2]Housefly (Musca domestica) and Blow Fly (Protophormia terraenovae) as Vectors of Bacteria Carrying Colistin Resistance Genes. Zhang, Jilei,Wang, Jiawei,Chen, Li,Yassin, Afrah Kamal,Li, Jing,Wang, Chengming,Yassin, Afrah Kamal,Kelly, Patrick,Butaye, Patrick,Butaye, Patrick,Gong, Jiansen,Cattley, Russell,Wang, Chengming,Qi, Kezong. 2018

[3]Identification and characterization of mcr mediated colistin resistance in extraintestinal Escherichia coli from poultry and livestock in China. Yassin, Afrah Kamal,Zhang, Jilei,Wang, Jiawei,Chen, Li,Lu, Guangwu,Li, Min,Wang, Yaoyao,Wang, Chengming,Yassin, Afrah Kamal,Kelly, Patrick,Butaye, Patrick,Butaye, Patrick,Gong, Jiansen,Wei, Lanjing,Qi, Kezong,Han, Xiangan,Price, Stuart,Hathcock, Terri,Wang, Chengming. 2017

[4]Characterization of NDM-5-positive extensively resistant Escherichia coli isolates from dairy cows. He, Tao,Wei, Ruicheng,Zhang, Lili,Sun, Lichang,Pang, Maoda,Wang, Ran,Wang, Yang.

[5]Generation of Newly Discovered Resistance Gene mcr-1 Knockout in Escherichia coli Using the CRISPR/Cas9 System. Sun, Lichang,He, Tao,Zhang, Lili,Pang, Maoda,Zhou, Yan,Bao, Hongduo,Wang, Ran,Zhang, Qiaoyan.

[6]Pathogenicity of Klebsiella pneumonia (KpC4) infecting maize and mice. Huang Min,He Peng-fei,He Peng-bo,Yuan Yuan,He Yue-qiu,Huang Min,Lin Li,Huang Min,Lin Li,Wu Yi-xin,Ho, Honhing,Li Guo-zhi,Xiong Guo-ru,He Yue-qiu. 2016

[7]The Klebsiella pneumoniae nitrogenase Fe protein gene (nifH) functionally substitutes for the chlL gene in Chlamydomonas reinhardtii. Cheng, Q,Day, A,Dowson-Day, M,Shen, GF,Dixon, R. 2005

[8]Complete genome sequence of endophytic nitrogen-fixing Klebsiella variicola strain DX120E. Lin, Li,Li, Yangrui,Yang, Litao,Lin, Li,Li, Yangrui,Yang, Litao,Lin, Li,Li, Yangrui,Yang, Litao,Lin, Li,Li, Yangrui,Yang, Litao,Wei, Chunyan,Li, Yangrui,Yang, Litao,Chen, Mingyue,Wang, Hongcheng,Li, Yuanyuan,An, Qianli,Yang, Litao. 2015

[9]Isolation and characterization of pyrene and benzo[a]pyrene-degrading Klebsiella pneumonia PL1 and its potential use in bioremediation. Ping, Lifeng,Zhang, Chunrong,Zhang, Changpeng,Zhu, Yahong,He, Hongmei,Wu, Min,Tang, Tao,Li, Zhen,Zhao, Hua,Ping, Lifeng,Zhang, Chunrong,Zhang, Changpeng,Zhu, Yahong,He, Hongmei,Wu, Min,Tang, Tao,Li, Zhen. 2014

[10]Characterization of antimicrobial resistance in Klebsiella species isolated from chicken broilers. Wu, Hao,Wang, Mingyu,Xu, Hai,Liu, Yuqing,Wang, Xinhua,Wang, Yunkun,Lu, Jinxing.

[11]Antimicrobial resistance in Escherichia coli isolates from food animals, animal food products and companion animals in China. Lei, Tao,Tian, Wei,He, Liu,Huang, Xian-Hui,Sun, Yong-Xue,Deng, Yu-Ting,Sun, Yan,Liu, Jian-Hua,Lv, Dian-Hong,Wu, Cong-Ming,Shen, Jian-Zhong,Huang, Liang-Zong. 2010

[12]High prevalence and widespread distribution of multi-resistant Escherichia coli isolates in pigs and poultry in China. Jiang, Hong-Xia,Chen, Zhang-Liu,Wang, Xiu-Mei,Chen, Ji-Rong,Liu, Ya-Hong,Liao, Xiao-Ping,Liu, Jian-Hua,Zeng, Zhen-Ling,Lue, Dian-Hong. 2011

[13]Paving the way to construct a new vaccine against Escherichia coli from its recombinant outer membrane protein C via a murine model. Wang, Xiao,Guan, Qingfeng,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Wang, Jianhua,Wang, Xiao,Yao, Junhu,Wang, Xiao,Guan, Qingfeng,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Wang, Jianhua. 2015

[14]Evaluation of Sanitizing Methods for Reducing Microbial Contamination on Fresh Strawberry, Cherry Tomato, and Red Bayberry. Wei, Wei,Wang, Xu,Xie, Zhongwen,Zhou, Yu,Wei, Wei,Wang, Wen,Xu, Junfeng,Liu, Yuanjing,Gao, Haiyan. 2017

[15]Comparison of base substitutions in response to nitrogen ion implantation and Co-60-gamma ray irradiation in Escherichia coli. Xie, CX,Xu, A,Wu, LJ,Yao, JM,Yang, JB,Yu, ZL. 2004

[16]Cloning, expression and characterization of a cold-adapted endo-1, 4-beta-glucanase from Citrobacter farmeri A1, a symbiotic bacterium of Reticulitermes labralis. Bai, Xi,Yuan, Xianjun,Li, Junfeng,Shao, Tao,Wen, Aiyou,Bai, Yunfeng. 2016

[17]An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species. Gomaa, Ahmed E.,Zhang, Chen,Yang, Zhimin,Shang, Liguo,Jiang, Shijie,Deng, Zhiping,Zhan, Yuhua,Lu, Wei,Lin, Min,Yan, Yongliang. 2017

[18]High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway. Fang, Ming-Yue,Zhang, Chong,Xing, Xin-Hui,Yang, Song,Cui, Jin-Yu,Jiang, Pei-Xia,Lou, Kai,Wachi, Masaaki. 2015

[19]Inhibition of Citrate Synthase Thermal Aggregation In Vitro by Recombinant Small Heat Shock Proteins. Wan, Fanghao,Guo, Jianying,Gong Weina,Xie, Bingyan,Yue, Ming. 2009

[20]Improvement of an E. coli bioreporter for monitoring trace amounts of phenol by deletion of the inducible sigma(54)-dependent promoter. Peng, Zixin,Yan, Yongliang,Xu, Yuquan,Yu, Haiying,Zhan, Yuhua,Zhang, Wei,Lin, Min,Chen, Ming,Takeo, Masahiro,Zhao, Zhonglin. 2010

作者其他论文 更多>>

微信小程序