MdVHA-A encodes an apple subunit A of vacuolar H+-ATPase and enhances drought tolerance in transgenic tobacco seedlings

文献类型: 外文期刊

第一作者: Dong, Qing-Long

作者: Dong, Qing-Long;Wang, Chun-Rong;Liu, Dan-Dan;Hu, Da-Gang;Fang, Mou-Jing;You, Chun-Xiang;Yao, Yu-Xin;Hao, Yu-Jin;Dong, Qing-Long

作者机构:

关键词: Apple (Malus domestica B.);Vacuolar H+-ATPase gene;Gene expression;Lateral root;Drought tolerance

期刊名称:JOURNAL OF PLANT PHYSIOLOGY ( 影响因子:3.549; 五年影响因子:4.164 )

ISSN: 0176-1617

年卷期: 2013 年 170 卷 6 期

页码:

收录情况: SCI

摘要: Vacuole H+-ATPases (VHAs) are plant proton pumps, which play a crucial role in plant growth and stress tolerance. In the present study, we demonstrated that the apple vacuolar H+-ATPase subunit A (MdVHA-A) is highly conserved with subunit A of VHA (VHA-A) proteins from other plant species. MdVHA-A was expressed in vegetative and reproductive organs. In apple in vitro shoot cultures, expression was induced by polyethylene glycol (PEG)-mediated osmotic stress. We further verified that over-expression of MdVHA-A conferred transgenic tobacco seedlings with enhanced vacuole H+-ATPase (VHA) activity and improved drought tolerance. The enhanced PEG-mimic drought response of transgenic tobacco seedlings was related to an extended lateral root system (dependent on auxin translocation) and more efficient osmotic adjustment. Our results indicate that MdVHA-A is a candidate gene for improving drought tolerance in plants. (C) 2013 Elsevier GmbH. All rights reserved.

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