Concurrent highly pathogenic porcine reproductive and respiratory syndrome virus infection accelerates Haemophilus parasuis infection in conventional pigs

文献类型: 外文期刊

第一作者: Wang, Jinbao

作者: Wang, Jinbao;Yu, Jiang;Wang, Jinbao

作者机构:

关键词: Effect;Co-infection;HP-PRRSV;Haemophilus parasuis;Quantitative real-time PCR

期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )

ISSN: 0378-1135

年卷期: 2012 年 158 卷 3-4 期

页码:

收录情况: SCI

摘要: This study was aimed at determining the effect of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) on Haemophilus parasuis (HPS) in co-infection. A quantitative real-time PCR targeting infB gene, which is conserved among different HPS serotypes, was developed to improve the accuracy and speed of the detection of HPS. A total of 32 four-week-old conventional pigs were distributed randomly into four groups: pigs in group I were intranasally infected with HP-PRRSV first, and were then intraperitoneally inoculated with HPS on 5 days after HP-PRRSV infection; pigs in group II were intranasally inoculated with HP-PRRSV alone; pigs in group III were intraperitoneally inoculated with HPS alone; pigs in group IV were intraperitoneally inoculated with physiological saline. The amount of HPS in serum on 0, 3, 6, 9 and 12 days post-inoculation (dpi) with HPS were detected using the established quantitative real-time PCR. Clinical signs, pathological changes and histopathological lesions were observed. The amount of HPS in serum reached 10(6) copies/mu l at 3 dpi with HPS in pigs of group I, while it arrived 10(5.7) copies/mu l at 9 dpi with HPS in pigs of group III. The HPS loads in hearts and lungs were much higher than in other tissues. The study showed that HP-PRRSV was able to accelerate BPS infection and loads. (C) 2012 Elsevier B.V. All rights reserved.

分类号:

  • 相关文献

[1]Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses. Li, Jiangnan,Wang, Shengnan,Li, Changyao,Wang, Chunlai,Liu, Yonggang,Wang, Gang,He, Xijun,Hu, Liang,Liu, Yuanyuan,Cui, Mengmeng,Bi, Caihong,Shao, Zengyu,Cai, Xuehui,Huang, Li,Weng, Changjiang,Wang, Xiaojie,Xiong, Tao.

[2]Co-Infection with Marek's Disease Virus and Reticuloendotheliosis Virus Increases Illness Severity and Reduces Marek's Disease Vaccine Efficacy. Sun, Guo-Rong,Zhang, Yan-Ping,Zhou, Lin-Yi,Lv, Hong-Chao,Zhang, Feng,Li, Kai,Gao, Yu-Long,Qi, Xiao-Le,Cui, Hong-Yu,Wang, Yong-Qiang,Gao, Li,Pan, Qing,Wang, Xiao-Mei,Liu, Chang-Jun. 2017

[3]Porcine bocaviruses: genetic analysis and prevalence in Chinese swine population. Zhang, H. B.,Huang, L.,Lin, T.,Sun, C. Q.,Deng, Y.,Wei, Z. Z.,Long, J. X.,Yuan, S. S.,Zhang, H. B.,Liu, Y. J.,Lin, T.,Cheung, A. K.. 2011

[4]Establishment of a Multiplex PCR and an Investigation of Co-Infection Rate of WSSV and IHHNV in Penaeid vannamei in Northern of Jiangsu. Chen, Yi-Bing,Gao, Song,Zhou, Jun-Fang,Wan, Xi-He. 2012

[5]Co-infection of H9N2 subtype avian influenza virus and infectious bronchitis virus decreases SP-A expression level in chickens. Huang, Qi,Wang, Kai,Pan, Ling,Qi, Kezong,Liu, Hongmei,Huang, Qi,Wang, Kai,Chen, Hongjun.

[6]The Cap Snatching of Segmented Negative Sense RNA Viruses as a Tool to Map the Transcription Start Sites of Heterologous Co-infecting Viruses. Lin, Wenzhong,Qiu, Ping,Jin, Jing,Liu, Shunmin,Ul Islam, Saif,Zhang, Jie,Du, Zhenguo,Wu, Zujian,Yang, Jinguang,Kormelink, Richard,Du, Zhenguo,Wu, Zujian. 2017

[7]The effects of Mycoplasma hyopneumoniae on porcine circovirus type 2 replication in vitro PK-15 cells. Wang, Haiyan,Feng, Zhixin,Wu, Yuzi,Wei, Yanna,Gan, Yuan,Hua, Lizhong,Li, Bin,Wang, Xiaomin,Liu, Maojun,Xiong, Qiyan,Shao, Guoqing,Wang, Haiyan,Feng, Zhixin,Shao, Guoqing.

[8]The occurrence of porcine circovirus 3 without clinical infection signs in Shandong Province. Zheng, S.,Wu, X.,Zhang, L.,Xin, C.,Shi, J.,Peng, Z.,Xu, S.,Yu, J.,Sun, W.,Xu, S.,Li, J.,Wang, J.,Zheng, S.,Liu, Y.,Fu, F..

[9]Transcription analysis on response of porcine alveolar macrophages to co-infection of the highly pathogenic porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae. Li, Bin,Du, Luping,Xu, Xiangwei,Sun, Bing,Yu, Zhengyu,Feng, Zhixin,Liu, Maojun,Wei, Yanna,Wang, Haiyan,Shao, Guoqing,He, Kongwang,Li, Bin,Yu, Zhengyu,Feng, Zhixin,Liu, Maojun,Wei, Yanna,Wang, Haiyan,Shao, Guoqing,He, Kongwang.

[10]Optimization of trans lutein from pumpkin (Cucurbita moschata) peel by ultrasound-assisted extraction. Song, Jiangfeng,Yang, Qiuming,Huang, Wuyang,Xiao, Yadong,Li, Dajing,Liu, Chunquan,Yang, Qiuming. 2018

[11]Insulation grapes cold winter effect. Guo Shao-jie,Li Ming. 2013

[12]Removal of ammonium-N from ammonium-rich sewage using an immobilized Bacillus subtilis AYC bioreactor system. Xiao, Jingjing,Zhu, Changxiong,Sun, Dongyuan,Guo, Ping,Tian, Yunlong.

[13]Subcellular localization of Bombyx mori ribosomal protein S3a and effect of its over-expression on BmNPV infection. Zhou Wu-song,Xu Jia-ping,You Zheng-ying,Yang Ying,Bao Xian-xun,Wu Hui-ling,Wang Wen-bing. 2010

[14]SO2, NOx and acid deposition problems in China impact on agriculture. Yang, L,Stulen, I,De Kok, LJ,Zheng, Y. 2002

[15]Process Optimization of Extraction and Separation of Water-soluble Crude Polysaccharide in Sweet Corn Cob. Song, Jiang-Feng,Liu, Chun-Quan,Li, Da-Jing.

[16]Pathological and immunological characteristics of piglets infected experimentally with a HP-PRRSV TJ strain. Li, Zhenguang,He, Yanliang,Li, Shufen,Xia, Mingqi,Wu, Hua,Li, Zhenguang,Leng, Xue,Wen, Yongjun,Wang, Fengxue,Cheng, Shipeng,Wu, Hua,Xu, Xiaoqin. 2016

[17]Identification of porcine serum proteins modified in response to HP-PRRSV HuN4 infection by two-dimensional differential gel electrophoresis. An, Tongqing,Gong, Daqing,Peng, Jinmei,Leng, Chaoliang,Yuan, Zhonghua,Tian, Zhijun,Yang, Yongqian,Li, Dengyun,Zhang, Deli,Tong, Guangzhi. 2012

[18]Mutations in the genome of the highly pathogenic porcine reproductive and respiratory syndrome virus potentially related to attenuation. Leng, Xue,Li, Zhenguang,Wang, Fengxue,Wang, Wei,Zhang, Xiuhua,Wu, Hua,Xia, Mingqi,Li, Xiyu,Wu, Hua.

[19]Characterizing the thymic lesions in piglets infected with attenuated strains of highly pathogenic porcine reproductive and respiratory syndrome virus. Wang, Gang,Yu, Ying,Tu, Yabin,Li, Yuming,Tong, Jie,Zhang, Chong,Liu, Yonggang,Li, Aidong,Jiang, Chenggang,Wang, Shujie,Tao, Ye,Cai, Xuehui,Zhang, Chong.

[20]Pathogenicity and Distribution of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in Pigs. Hu, S. P.,Zhang, Z.,Liu, Y. G.,Tian, Z. J.,Wu, D. L.,Cai, X. H.,He, X. J.. 2013

作者其他论文 更多>>