Cloning and analysis of a NBS-LRR disease resistance gene candidate PnAG(1) from peanut (Arachis hypogaea L.)
文献类型: 外文期刊
第一作者: Wan, Shu-bo
作者: Wan, Shu-bo;Shan, Shi-hua;Zhang, Ting-ting;Li, Chun-juan;Yang, Chen;Yan, Cai-xia;Yang, Chen
作者机构:
关键词: bioinformatics;NBS-LRR;peanut;real-time fluorescence quantitative PCR
期刊名称:ELECTRONIC JOURNAL OF BIOTECHNOLOGY ( 影响因子:2.8; 五年影响因子:3.379 )
ISSN: 0717-3458
年卷期: 2011 年 14 卷 6 期
页码:
收录情况: SCI
摘要: Background: Based on the conserved sequences of a known NBS resistance gene, a pair of degenerate primers was designed to amplify the NBS-LRR resistance gene from peanut using PCR and RACE methods. Results: Analyzing the amino acid sequence by BLAST on NCBI, which was deduced from the 1088bp-long gene named PnAG1-2, showed that it had a certain homology with some resistance proteins, among which Arachis cardenasii resistance protein gene had the highest homology (66%). Relative quantification PCR analysis indicated that PnAG1-2 gene expresses more in J11 (an A. flavus-resistant variety) than in JH1012 (an A. flavus-susceptible variety) when the harvest time was coming. Conclusions: In this study, the NBS-LRR resistance sequence was successfully cloned from peanut and prokaryotic expression was done on the gene, which provided a foundation for cultivating anti-A. flavus peanut varieties.
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