A Study of Miriplatin and Its Pt-metabolites in Beagle Dog Plasma by Size Exclusion Chromatography-Inductively Coupled Plasma Mass Spectrometry

文献类型: 外文期刊

第一作者: Liu De-Ye

作者: Liu De-Ye;Huo Zong-Li;Liu Hua-Liang;Hao Yuan-Bin;Li Jian;Han Wen-Ru

作者机构:

关键词: Miriplatin;Platinum-metabolites;Speciation;Pharmacokinetics

期刊名称:CHINESE JOURNAL OF ANALYTICAL CHEMISTRY ( 影响因子:1.134; 五年影响因子:0.909 )

ISSN: 0253-3820

年卷期: 2014 年 42 卷 11 期

页码:

收录情况: SCI

摘要: A study of Pt-metabolites from a new anti. hepatoma drug miriplatin was important to miriplatin's pharmacology research. Therefore, a method based on size exclusion chromatography. inductively coupled plasma mass spectrometry (SEC-ICP-MS) was developed to study miriplatin and its Pt-metabolites in Beagle dog plasma. This method could be used to study total platinum concentration half. quantitatively. Compared with traditional ICP-MS direct determination, data acquired from this SEC-ICP-MS method were almost the same. By using BioSep-s2000 column, 25 mmol/L of pH 7.2 phosphate buffer as eluent, and Pt-195 as detecting isotope, we discovered miriplatin with its four Pt-metabolites in dog plasma after intra. hepatic artery administration. The main Pt-metabolite was m(2), which associated with plasma proteins. Miriplatin in plasma did not bind with plasma proteins. According to calculation, the ratio of miriplatin/m(2) first decreased rapidly, and then slowly increased to its second climax, finally slowly decreased.

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