A novel functional gene associated with cold tolerance at the seedling stage in rice

文献类型： 外文期刊

第一作者： Zhao, Junliang

作者： Zhao, Junliang;Zhang, Shaohong;Dong, Jingfang;Yang, Tifeng;Mao, Xingxue;Liu, Qing;Wang, Xiaofei;Liu, Bin;Zhao, Junliang;Zhang, Shaohong;Dong, Jingfang;Yang, Tifeng;Mao, Xingxue;Liu, Qing;Wang, Xiaofei;Liu, Bin

作者机构：

关键词： rice;quantitative trait locus (QTL);cold tolerance;QTL mapping;expression profiling;gene cloning

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

年卷期： 2017 年 15 卷 9 期

页码：

收录情况： SCI

摘要： Identification and cloning of cold-tolerant genes that can stably express under different cold environments are crucial for molecular rice breeding for cold tolerance. In the previous study, we identified a cold-tolerant QTL at the seedling stage, qCTS-9 which could be detected under different cold environments using a recombinant inbred line (RIL) population derived from a cold-tolerant variety Lijiangxintuanheigu (LTH) and a cold-sensitive variety Shanhuangzhan 2 (SHZ-2). In this study, eight candidate genes within the qCTS-9 interval were identified through integrated analysis of QTL mapping with genomewide differential expression profiling of LTH. The qRT-PCR assay showed that only Os09g0410300 exhibited different expression patterns between LTH and SHZ-2 during cold stress, and significantly positive correlation was found between cold induction of Os09g0410300 and seedling cold tolerance in the RI lines. Five SNPs and one InDel in the promoters of Os09g0410300 were detected between LTH and SHZ-2, and the InDel marker ID410300 designed based on the insertion-deletion polymorphism in the promoter was significantly associated with seedling cold tolerance in RIL population. Further, Os09g0410300 over-expression plants exhibited enhanced cold tolerance at the seedling stage compared with the wild-type plants. Thus, our results suggest that Os09g0410300 is the functional gene underlying qCTS-9. To our knowledge, it is a novel gene contributed to enhance cold tolerance at the seedling stage in rice. Identification of the functional gene underlying qCTS-9 and development of the gene-specific marker will facilitate molecular breeding for cold tolerance at the seedling stage in rice through transgenic approach and marker-assisted selection (MAS).

分类号：

  • 相关文献

[1]Genetic Analysis of Cold Tolerance at Seedling Stage and Heat Tolerance at Anthesis in Rice (Oryza sativa L.). Cheng Li-rui,Uzokwe, Veronica,Meng Li-jun,Wang Yun,Sun Yong,Zhu Ling-hua,Xu Jian-long,Li Zhi-kang,Wang Jun-min,Li Zhi-kang. 2012

[2]Identification and pyramiding of QTLs for cold tolerance at the bud bursting and the seedling stages by use of single segment substitution lines in rice (Oryza sativa L.). Yang, Tifeng,Zhang, Shaohong,Zhao, Junliang,Liu, Qing,Huang, Zhanghui,Mao, Xingxue,Dong, Jingfang,Wang, Xiaofei,Liu, Bin,Yang, Tifeng,Zhang, Guiquan,Yang, Tifeng,Zhang, Shaohong,Zhao, Junliang,Liu, Qing,Huang, Zhanghui,Mao, Xingxue,Dong, Jingfang,Wang, Xiaofei,Liu, Bin.

[3]Identification of QTLs for cold tolerance at seedling stage in rice (Oryza sativa L.) using two distinct methods of cold treatment. Zhang, Shaohong,Liu, Bin,Zhao, Junliang,Wang, Xiaofei,Yang, Tifeng,Huang, Zhanghui,Zheng, Jingsheng,Peng, Shaobing,Leung, Hei. 2014

[4]QTL mapping for the paste viscosity characteristics in rice (Oryza sativa L.). Bao, JS,Zheng, XW,Xia, YW,He, P,Shu, QY,Lu, X,Chen, Y,Zhu, LH. 2000

[5]Identification of salt-tolerant QTLs with strong genetic background effect using two sets of reciprocal introgression lines in rice. Cheng, Lirui,Wang, Yun,Meng, Lijun,Hu, Xia,Cui, Yanru,Sun, Yong,Zhu, Linghua,Xu, Jianlong,Li, Zhikang,Wang, Yun,Ali, Jauhar,Li, Zhikang.

[6]Identification of QTLs associated with salt or alkaline tolerance at the seedling stage in rice under salt or alkaline stress. Liang, Jing-long,Zhao, Zheng-wu,Zhang, Tao,Liang, Jing-long,Qu, Ying-ping,Ma, Xiao-ding,Cao, Gui-lan,Han, Long-zhi,Yang, Chun-gang,Zhang, San-yuan.

[7]Quantitative trait loci analysis for rice seed vigor during the germination stage. Wang, Zhou-fei,Wang, Jian-fei,Bao, Yong-mei,Wang, Fu-hua,Zhang, Hong-sheng,Wang, Fu-hua. 2010

[8]Identification of quantitative trait loci for the dead leaf rate and the seedling dead rate under alkaline stress in rice. Qi, Dongling,Cao, Guilan,Han, Longzhi,Qi, Dongling,Guo, Guizhen,Zhang, Junguo,Zhang, Sanyuan,Han, Longzhi,Lee, Myung-Chul,Suh, Seok-Cheol,Zhou, Qingyang. 2008

[9]Knock out of the annexin gene OsAnn3 via CRISPR/Cas9-mediated genome editing decreased cold tolerance in rice. Tang, Ning,Cao, Mengliang,Shen, Chunxiu,Que, Zhiqun,Cao, Mengliang,Li, Ding,He, Ronghua. 2017

[10]Genome-wide gene expression profiling of introgressed indica rice alleles associated with seedling cold tolerance improvement in a japonica rice background. Zhang, Fan,Huang, Liyu,Wang, Wensheng,Zhao, Xiuqin,Zhu, Linghua,Fu, Binying,Li, Zhikang,Zhang, Fan,Huang, Liyu,Wang, Wensheng,Zhao, Xiuqin,Zhu, Linghua,Fu, Binying,Li, Zhikang,Li, Zhikang. 2012

[11]Quantitative trait loci for cold tolerance of rice recombinant inbred lines in low temperature environments. Jiang, Wenzhu,Pan, Hong-Yu,Du, Xinglin,Jin, Yong-Mei,Lee, Joohyun,Lee, Kang-Ie,Piao, Rihua,Koh, Hee-Jong,Jin, Yong-Mei,Lee, Joohyun,Lee, Kang-Ie,Piao, Rihua,Koh, Hee-Jong,Han, Longzhi,Shin, Jin-Chul,Jin, Rong-De,Cao, Tiehua. 2011

[12]Overexpression of the OsIMP Gene Increases the Accumulation of Inositol and Confers Enhanced Cold Tolerance in Tobacco through Modulation of the Antioxidant Enzymes' Activities. Zhang, Rong-Xiang,Qin, Li-Jun,Zhao, De-Gang,Zhang, Rong-Xiang,Qin, Li-Jun,Zhao, De-Gang,Zhang, Rong-Xiang,Zhao, De-Gang,Zhao, De-Gang,Zhang, Rong-Xiang. 2017

[13]MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice. Yu, Haiping,Zhu, Yangzhou,Xu, Jiangmin,Lu, Mei,Ma, Bojun,Rao, Yuchun,Yu, Haiping,Ren, Deyong,Zhu, Yangzhou,Wang, Yuexing,Liu, Ruifang,Fang, Yunxia,Shi, Zhenyuan,Pan, Jiangjie,Hu, Jiang,Rao, Yuchun,Yu, Haiping,Yu, Haiping. 2016

[14]A Kelch Motif-Containing Serine/Threonine Protein Phosphatase Determines the Large Grain QTL Trait in Rice. Hu, Zejun,Sun, Fan,Xin, Xiaoyun,Qian, Xi,Yang, Jingshui,Luo, Xiaojin,Hu, Zejun,He, Haohua,Wang, Wenxiang,Zhang, Shiyong. 2012

[15]Identification of QTL Associated with Nitrogen Uptake and Nitrogen Use Efficiency Using High Throughput Genotyped CSSLs in Rice (Oryza sativa L.). Zhou, Yong,Tao, Yajun,Tang, Dongnan,Zhong, Jun,Wang, Yi,Yuan, Qiumei,Yu, Xiaofeng,Zhang, Yan,Wang, Yulong,Liang, Guohua,Dong, Guichun,Wang, Jun. 2017

[16]Fine Mapping of qTGW3-1, a QTL for 1 000-Grain Weight on Chromosome 3 in Rice. Zhang Qiang,Yao Guo-xin,Hu Guang-long,Chen Chao,Tang Bo,Zhang Hong-liang,Li Zi-chao,Zhang Qiang,Yao Guo-xin. 2012

[17]Integrating Small RNA Sequencing with QTL Mapping for Identification of miRNAs and Their Target Genes Associated with Heat Tolerance at the Flowering Stage in Rice. Liu, Qing,Yang, Tifeng,Zhang, Shaohong,Mao, Xingxue,Zhao, Junliang,Wang, Xiaofei,Dong, Jingfang,Liu, Bin,Liu, Qing,Yang, Tifeng,Zhang, Shaohong,Mao, Xingxue,Zhao, Junliang,Wang, Xiaofei,Dong, Jingfang,Liu, Bin,Yu, Ting. 2017

[18]QTL mapping of grain weight in rice and the validation of the QTL qTGW3.2. Shao-qing, Tang,Gao-neng, Shao,Xiang-jin, Wei,Ming-liang, Chen,Zhong-hua, Sheng,Ju, Luo,Gui-ai, Jiao,Li-hong, Xie,Pei-song, Hu.

[19]Identification of QTLs associated with tissue culture response through sequencing-based genotyping of RILs derived from 93-11 x Nipponbare in rice (Oryza sativa). Li, Sujuan,Yan, Song,Zou, Guihua,Tao, Yuezhi,Li, Sujuan,Qian, Qian,Wang, A-hong,Huang, Xuehui,Han, Bin,Qian, Qian.

[20]Dissecting the genetic basis for the effect of rice chalkiness, amylose content, protein content, and rapid viscosity analyzer profile characteristics on the eating quality of cooked rice using the chromosome segment substitution line population across eight environments. Liu, Xiaolu,Wan, Jianmin,Liu, Xiaolu,Wan, Jianmin,Wan, Xiangyuan,Ma, Xiaodong,Wan, Jianmin,Wan, Xiangyuan.

作者其他论文 更多>>

微信小程序