Production of functional human nerve growth factor from the saliva of transgenic mice by using salivary glands as bioreactors

文献类型： 外文期刊

第一作者： Zeng, Fang

作者： Zeng, Fang;Li, Zicong;Zhu, Qingchun;Dong, Rui;Zhao, Chengcheng;Li, Guoling;Li, Guo;Gao, Wenchao;Jiang, Gelong;Zheng, Enqin;Cai, Gengyuan;Yang, Huaqiang;Liu, Dewu;Wu, Zhenfang;Zeng, Fang;Li, Zicong;Zhu, Qingchun;Dong, Rui;Zhao, Chengcheng;Li, Guoling;Li, Guo;Gao, Wenchao;Jiang, Gelong;Zheng, Enqin;Cai, Gengyuan;Yang, Huaqiang;Liu, Dewu;Wu, Zhenfang;Moisyadi, Stefan;Urschitz, Johann;Moisyadi, Stefan

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： The salivary glands of animals have great potential to act as powerful bioreactors to produce human therapeutic proteins. Human nerve growth factor (hNGF) is an important pharmaceutical protein that is clinically effective in the treatment of many human neuronal and non-neuronal diseases. In this study, we generated 18 transgenic (TG) founder mice each carrying a salivary gland specific promoter-driven hNGF transgene. A TG mouse line secreting high levels of hNGF protein in its saliva (1.36 mu g/mL) was selected. hNGF protein was successfully purified from the saliva of these TG mice and its identity was verified. The purified hNGF was highly functional as it displayed the ability to induce neuronal differentiation of PC12 cells. Furthermore, it strongly promoted proliferation of TF1 cells, above the levels observed with mouse NGF. Additionally, saliva collected from TG mice and containing unpurified hNGF was able to significantly enhance the growth of TF1 cells. This study not only provides a new and efficient approach for the synthesis of therapeutic hNGF but also supports the concept that salivary gland from TG animals is an efficient system for production of valuable foreign proteins.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序