Characterization and functional analysis of small heat shock protein gene HSP20.1 in Bombyx mandarina

文献类型： 外文期刊

第一作者： An, Hui

作者： An, Hui;Ding, Jiaqi;Chen, Enxi;Li, Ruonan;Lin, Mingjun;Qian, Heying;Zhao, Guodong;Ding, Jiaqi;Chen, Enxi;Li, Ruonan;Lin, Mingjun;Qian, Heying;Zhao, Guodong

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关键词： Bombyx mandarina; Small heat shock protein gene; Expression analysis; External stress; RNA interference

期刊名称：INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE （ 影响因子：1.2； 五年影响因子：1.3 ）

ISSN： 1742-7584

年卷期： 2024 年 44 卷 6 期

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收录情况： SCI

摘要： Heat shock proteins (HSPs) act as molecular chaperones and are widely distributed in all kinds of organisms. In this study, the small heat shock protein HSP20.1 gene was cloned from Bombyx mandarina. Its open reading frame (ORF) is 537 bp in length, encoding 178 amino acids with the sHSP characteristic domain. The relative molecular weight of the encoding protein was 20.1 kD and the isoelectric point (pI) is 5.46. Homologous comparison and phylogenetic analysis showed that the insect sHSP protein is highly conservative. BmmHSP20.1 has the closest relationship with BmHSP20.1 of Bombyx mori. The results of real-time quantitative PCR showed that BmmHSP20.1 was highly expressed in the anterior, middle and posterior silk glands of the 5th instar larvae. The expression level of BmmHSP20.1 gene was increased in all tissues under high (40 degrees C) and low (8 degrees C) temperature stress. Bombyx mori nucleopolyhedrovirus (BmNPV) can induce the expression of BmmHSP20.1 gene in hemolymph and midgut. The expression of BmmHSP20.1 gene was up-regulated in the midgut of larvae under deltamethrin stress at room temperature (25 degrees C). Larval RNA interference of BmmHSP20.1caused 29.2% insect mortality. The whole cocoon weight, cocoon shell rate, oviposition amount and unfertilized rate had no significant differences. The results suggested BmmHSP20.1 gene may play an important role in the molecular mechanism of response to external stress and development in B. mandarina.

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