Comparative study of the promotion of porcine fetal fibroblast proliferation by overexpression of two transcriptional variants of SIRT6

文献类型： 外文期刊

第一作者： Xie XiaoXian

作者： Xie XiaoXian;He Kan;Wang QiShan;Ma YuFang;Xie XiaoXian;Zhang AiLing;Zhang Hao;Li JiaQi;Zhang AiLing;Zhang Hao;Li JiaQi

作者机构：

关键词： SIRT6 variant;overexpression;porcine fetal fibroblast;proliferation;prediction assay

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 2013 年 58 卷 10 期

页码：

收录情况： SCI

摘要： Mammalian Sirtuin 6 (SIRT6) plays an important role in the transcriptional silencing of silent mating-type loci, telomeres, and rDNA. However, the roles of porcine SIRT6 in cell proliferation are poorly understood, and a better knowledge of these will help improve our understanding of the biological mechanisms of cell growth and development. In this study, a novel variant of porcine SIRT6 (SIRT6 V2) identified by reverse transcription-polymerase chain reaction and BLAST analysis showed a 124-bp deletion compared to wild-type SIRT6 mRNA (SIRT6 V1). Two recombinant plasmids overexpressing SIRT6 V1 and SIRT6 V2 were produced and their roles in the proliferation of porcine fetal fibroblasts (PFFs) were compared. Cells transfected with SIRT6 V1 proliferated significantly faster than those transfected with SIRT6 V2 (P < 0.01), though both proliferated significantly faster than cells expressing an empty vector (P < 0.01). These results might be caused by altered proportions of alpha helices and beta sheets in the SIRT6 V2 structure relative to SIRT6 V1. These results indicate that overexpression of SIRT6 V1/V2 was involved in promoting PFF proliferation. Deletion of a 124-bp sequence attenuated the effects of SIRT6 on cell proliferation, possibly as a result of changes in the proportions of alpha helices and beta sheets in the protein secondary structure.

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