Regulation of protein turnover by L-glutamine in porcine intestinal epithelial cells

文献类型： 外文期刊

第一作者： Xi, Pengbin

作者： Xi, Pengbin;Jiang, Zongyong;Zheng, Chuntian;Lin, Yingcai;Xi, Pengbin;Dai, Zhaolai;Li, Xilong;Yao, Kang;Wang, Junjun;Wu, Guoyao;Xi, Pengbin;Dai, Zhaolai;Li, Xilong;Yao, Kang;Wang, Junjun;Wu, Guoyao;Yao, Kang;Wu, Guoyao;Wang, Junjun;Wu, Guoyao

作者机构：

关键词： Glutamine;Protein turnover;Intestinal cells

期刊名称：JOURNAL OF NUTRITIONAL BIOCHEMISTRY （ 影响因子：6.048； 五年影响因子：6.114 ）

ISSN： 0955-2863

年卷期： 2012 年 23 卷 8 期

页码：

收录情况： SCI

摘要： L-Glutamine (Gin) plays an important role in sustaining the intestinal mucosal mass of humans and animals. However, the underlying mechanisms are largely unknown. This study tested the hypothesis that Gin regulates protein turnover in intestinal epithelial cells. Intestinal porcine epithelial cells (IPEC-1) were cultured for 3 h (short-term study) or 96 h (long-term study) in Gin-free Dulbecco's modified Eagle-F12 Ham medium containing 0, 0.5 or 2.0 mM Gin. To determine effects of ammonia (a metabolite of Gln, i.e., 0.18 mM ammonia produced from 2 nnM Gln in 3 h) on protein turnover, additional experiments were conducted in which medium contained 0.5 mM Gln and 0, 0.2, 0.5 or 2.0 mM NH4Cl. Variables of analysis included cell growth, protein synthesis, proteolysis and mammalian target of rapamycin (mTOR) signaling. IPEC-1 cell growth increased with extracellular Gin concentrations. Compared with 0 mM Gin, the addition of 0.5 and 2 mM Gin to medium stimulated protein synthesis and inhibited protein degradation in those cells in both the short- and long-term studies. Ammonia (0.05 to 2.0 mM) did not affect protein synthesis, although higher levels of ammonia (0.5 and 2.0 mM) reduced protein degradation in IPEC-1 cells. Consistent with the data on protein turnover, 0.5 and 2 mM Gin increased abundance of phosphorylated elF4E-binding protein-1 and phosphorylated S6 kinase-1 proteins. Collectively, these results demonstrate that physiological levels of Gin regulate protein turnover independent of ammonia production in intestinal cells through the mTOR signaling pathway. (C) 2012 Elsevier Inc. All rights reserved.

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