Molecular cloning and characterization of two novel NAC genes from Mikania micrantha (Asteraceae)

文献类型： 外文期刊

第一作者： Li, D. M.

作者： Li, D. M.;Zhu, G. F.;Lu, F. B.;Wang, J. H.;Peng, S. L.

作者机构：

关键词： Mikania micrantha;NAC genes;Sequence characterization;Transcript expression

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2012 年 11 卷 4 期

页码：

收录情况： SCI

摘要： NAC proteins, which are plant-specific transcription factors, have been identified to play important roles in plant response to stresses and in plant development. The full-length cDNAs that encode 2 putative NAC proteins, designated as MmATAF1 and MmNAP, respectively, were cloned from Mikania micrantha by rapid amplification of cDNA ends. The full-length cDNAs of MmATAF1 and MmNAP were 1329 and 1072 bp, respectively, and they encoded deduced proteins of 260- and 278-amino acid residues, respectively. The proteins MmATAF1 and MmNAP had a calculated molecular mass of 29.81 and 32.55 kDa and a theoretical isoelectric point of 7.08 and 9.00, respectively. Nucleotide sequence data indicated that both MmATAF1 and MmNAP contained 2 introns and 3 exons and that they shared a conserved genomic organization. Multiple sequence alignments showed that MmATAF1 showed high sequence identity with ATAF1 of Arabidopsis thaliana (61%) and that MmNAP showed high sequence identity with NAP of A. thaliana (67%) and CitNAC of Citrus sinensis Osbeck (62%). Phylogenetic analysis showed that the predicted MmATAF1 and MmNAP proteins were classified into the ATAF and NAP subgroups, respectively. Transient expression analysis of onion epidermal cells indicated nuclear localization of both MmATAF1-GFP and MmNAP-GFP fusion proteins. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis indicated that MmATAF1 was expressed in all the tissues tested, but in varying abundance, while MmNAP was specifically expressed in stems, petioles, shoots, and leaves, but not in roots. The transcript levels of MmATAF1 and MmNAP in shoots and in infected stems were induced and strengthened by wounding, exogenous ZnSO4, abscisic acid, salicylic acid, and Cuscuta campestris infection on the basis of semi-quantitative RT-PCR and real-time PCR analyses, respectively. Collectively, these results indicated that MmATAF1 and MmNAP, besides having roles in M. micrantha adaptation to C. campestris infection and abiotic stresses, also integrated signals derived from both C. campestris infection and abiotic stresses.

分类号：

  • 相关文献

[1]Expression Patterns, Activities and Carbohydrate-Metabolizing Regulation of Sucrose Phosphate Synthase, Sucrose Synthase and Neutral Invertase in Pineapple Fruit during Development and Ripening. Zhang, Xiu-Mei,Du, Li-Qing,Xie, Jiang-Hui,Yao, Yan-Li,Sun, Guang-Ming,Zhang, Xiu-Mei,Wang, Wei. 2012

[2]Suppression of reproductive characteristics of the invasive plant Mikania micrantha by sweet potato competition. Shen, Shicai,Xu, Gaofeng,Jin, Guimei,Liu, Shufang,Yang, Yanxian,Chen, Aidong,Zhang, Fudou,Clements, David Roy,Kato-Noguchi, Hisashi. 2016

[3]Tall grasses have an advantage over the invasive vine Mikania micrantha: Potential control agents. Zhou, Sheng,Li, Mingguang,Gao, Lei,Chen, Ping,Wu, Zhi,Xian, Wensheng,Fu, Weidong.

[4]Cloning, Sequence Characterization and Phylogenetic Analysis on Full-Length cDNA of Growth Hormone from Southern Flounder (Paralichthys lethostigma). Zang, Xiao-Nan,Liu, Shun-Mei,Zhang, Xue-Cheng,Liu, Bin,Lei, Ji-Lin. 2011

[5]Sequence characterization and promoter identification of porcine APC10 gene. He, W. B.,Peng, K. M.,He, W. B.,Wang, Z. W.,Li, Y.,Tang, Z. L.,Yang, S. L.,Mu, Y. L.,Peng, K. M.,Li, K..

[6]Molecular cloning, sequence characterization of a novel pepper gene NADP-ICDH and its effect on cytoplasmic male sterility. Dai, X. Z.,Zhang, Z. Q.,Zou, X. X.,Deng, M. H.,Zhu, H. S.,Zhou, H.,Wen, J. F.,Huo, J. L.. 2012

[7]Sequence characterization and expression patterns of two defensin-like antimicrobial peptides from the tick Haemaphysalis longicornis. Zhou, Jinlin,Liao, Min,Ueda, Marni,Gong, Haiyan,Xuan, Xuenan,Fujisaki, Kozo. 2007

作者其他论文 更多>>

微信小程序