Study on the Subcellular Localization of GAPDH in Rice (Oryza sativa)

文献类型： 外文期刊

第一作者： Su, Jing

作者： Su, Jing;Zhu, Xiao-yuan

作者机构：

关键词： Glyceraldehyde-3-phosphate dehydrogenase (GAPDH);Subcellular localization;Rice

期刊名称：BIOTECHNOLOGY, CHEMICAL AND MATERIALS ENGINEERING, PTS 1-3

ISSN： 1022-6680

年卷期： 2012 年 393-395 卷

页码：

收录情况： SCI

摘要： The glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is considered to be a housekeeping glycolytic enzyme that catalyzes an important energy-yielding step in carbohydrate metabolism in the cytoplasm. However, GAPDH participates in several other biological activities via the changes of its subcellular localization, which depends largely on the modifications of its catalytic active site Cys residue. In this study, we demonstrated that the active site mutation C154S induced the cytoplasmic accumulation of GAPDH, whereas the wild type was present mainly in the cytoplasm. However, the C-terminal truncated mutant of GAPC, GAPC Delta C-GFP was present mainly in nuclei in rice protoplast. The further interaction analysis indicated that the GAPC Delta C disrupted the dimeric GAPC formation, may thus, facilitating its nuclear localization. The result suggested that the nuclear translocation of GAPDH also depends on its oligomeric forms in rice.

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