Phylogenetic analysis of the citrus Huanglongbing (HLB) bacterium based on the sequences of 16S rDNA and 16S/23S rDNA intergenic regions among isolates in China
文献类型: 外文期刊
第一作者: Ding, Fang
作者: Ding, Fang;Hong, Ni;Wang, Guoping;Deng, Xiuxin;Zhong, Yun;Yi, Ganjun
作者机构:
关键词: Citrus Huanglongbing;16S rDNA;16S/23S rDNA;Phylogenetic analysis
期刊名称:EUROPEAN JOURNAL OF PLANT PATHOLOGY ( 影响因子:1.907; 五年影响因子:2.022 )
ISSN: 0929-1873
年卷期: 2009 年 124 卷 3 期
页码:
收录情况: SCI
摘要: Phylogenetic analysis of Chinese isolates of the citrus Huanglongbing (HLB) bacterium based on the 16S rDNA and 16S/23S rDNA intergenic regions sequences was carried out. Nine HLB samples collected from different hosts with different symptoms in seven Chinese provinces, were subjected to PCR for amplifying and sequencing the 16S rDNA. The identity level among Chinese isolates was 98.5% to 100% and was the same with the Indian HLB isolate 'Poona' (GenBank accession number: L22532). By contrast, identity values were 97.5% to 97.8% with Candidatus Liberibacter africanus strain 'Nelspruit' (L22533), 96.3% to 97.3% with Ca. L. africanus subsp. 'Capensis' (AF137368), 95.3% to 96.5% with the Ca. Liberibacter sp. 'LSg2' (AY919312), and 94.9% to 96.0% with a strain of Ca. L. americanus from Brazil (SA o pound Paulo State; AY742824). A phylogenetic tree constructed with 16S rDNA sequences showed that all Chinese isolates belong to Ca. L. asiaticum. Analysis of the 16S/23S rDNA intergenic region was conducted on 18 HLB-diseased citrus samples with different symptoms, collected in seven provinces. These isolates showed no obvious variation and had an identity level > 99.0% with one another. Sequence analysis of 16S/23S rDNA intergenic region and the relative phylogenetic tree showed that the Chinese isolates are very close to Ca. L. asiaticus, and distinct from Ca. L. africanus and Ca. L. americanus. These results suggest that the Chinese HLB isolates belong to the species Candidatus Liberibacter asiaticus. This is the first report on the classification of HLB isolates from China based on molecular investigations.
分类号:
- 相关文献
作者其他论文 更多>>
-
Phased genomics reveals hidden somatic mutations and provides insight into fruit development in sweet orange
作者:Wang, Nan;Chen, Peng;Xu, Yuanyuan;Guo, Lingxia;Li, Xianxin;Wang, Nan;Yi, Hualin;Larkin, Robert M.;Deng, Xiuxin;Xu, Qiang;Wang, Nan;Zhou, Yongfeng;Chen, Peng;Xu, Yuanyuan;Guo, Lingxia;Li, Xianxin;Yi, Hualin;Larkin, Robert M.;Deng, Xiuxin;Xu, Qiang;Zhou, Yongfeng
关键词:
-
High-quality genome assemblies for two Australimusa bananas (Musa spp.) and insights into regulatory mechanisms of superior fiber properties
作者:Zhou, Run;Zhan, Ni;He, Weidi;Deng, Guiming;Dou, Tongxin;Hu, Chunhua;Bi, Fangcheng;Gao, Huijun;Dong, Tao;Liu, Siwen;Li, Chunyu;Yang, Qiaosong;Yi, Ganjun;Sheng, Ou;Zhou, Run;Wang, Shuo;Zhu, Xi-Tong;Xie, Wen-Zhao;Zheng, Yu-Yu;Wang, Lingqiang;Song, Jia-Ming;Chen, Ling-Ling;Dang, Jiangbo;Guo, Qigao
关键词:Australimusabananasx; T genome; Musaceae evolution; structural variations; fiber
-
Identification and characterization of two P450 enzymes from Citrus sinensis involved in TMTT and DMNT biosyntheses and Asian citrus psyllid defense
作者:Sun, Xueli;Hu, Chunhua;Yi, Ganjun;Zhang, Xinxin;Sun, Xueli;Hu, Chunhua;Yi, Ganjun;Zhang, Xinxin;Sun, Xueli
关键词:
-
Soil water movement may regulate soil water consumption and improve cotton yields under different cotton cropping systems
作者:Wu, Fengqi;Guo, Simeng;Huang, Weibin;Wang, Zhanbiao;Feng, Lu;Li, Yabing;Han, Yingchun;Wang, Zhanbiao;Feng, Lu;Wang, Guoping;Li, Xiaofei;Lei, Yaping;Zhi, Xiaoyu;Xiong, Shiwu;Jiao, Yahui;Xin, Minghua;Yang, Beifang;Li, Yabing;Wu, Fengqi;Wu, Fengqi;Guo, Simeng;Li, Yabing;Huang, Weibin;Wang, Zhanbiao
关键词:Cotton; Yield; Soil water movement; Soil water consumption; Planting system
-
Quantifying physiological contributions to yield loss in response to planting date in short-season cotton under a cotton-wheat double-cropping system
作者:Guo, Simeng;Wu, Fengqi;Jia, Yaoyu;Li, Yabing;Feng, Lu;Han, Yingchun;Wang, Guoping;Chen, Jiale;Li, Xiaofei;Du, Wenli;Li, Yabing;Feng, Lu
关键词:Planting date; Physiological contributions; Yield loss; Short-season cotton
-
Transcriptional landscape of cotton roots in response to salt stress at single-cell resolution
作者:Li, Pengtao;Liu, Qiankun;Wei, Yangyang;Liu, Yuling;Lu, Quanwei;Hu, Nan;Wang, Tao;Cheng, Shuang;Li, Zhaoguo;Zhao, Zilin;Li, Yanfang;Peng, Renhai;Li, Pengtao;Liu, Qiankun;Xing, Chaozhu;Han, Jiangping;Cai, Xiaoyan;Zhou, Zhongli;Wang, Kunbo;Liu, Fang;Li, Pengtao;Xing, Chaozhu;Liu, Fang;Peng, Renhai;Liu, Qiankun;Cheng, Shuang;Li, Zhaoguo;Peng, Renhai;Xu, Zhongping;Zhu, Xiangqian;Jin, Shuangxia;Ding, Fang;Zhang, Baohong
关键词:cotton; salt stress; scRNA-seq; root cell types; virus-induced gene silencing
-
MaSMG7-Mediated Degradation of MaERF12 Facilitates Fusarium oxysporum f. sp. cubense Tropical Race 4 Infection in Musa acuminata
作者:Huang, Huoqing;Liu, Siwen;Huo, Yile;Liu, Yushan;Yi, Ganjun;Li, Chunyu;Liu, Siwen;Yi, Ganjun;Li, Chunyu;Liu, Siwen;Yi, Ganjun;Li, Chunyu;Tian, Yuzhen
关键词:banana wilt; Fusarium oxysporum f. sp. cubense tropical race 4; MaERF12; MaSMG7; cell death