Molecular Characterization of High Plant Species Using PCR with Primers Designed from Consensus Branch Point Signal Sequences

文献类型： 外文期刊

第一作者： Xiong, Faqian

作者： Xiong, Faqian;Jiang, Jing;Han, Zhuqiang;Zhong, Ruichun;He, Liangqiong;Tang, Ronghua;Zhuang, Weijian

作者机构：

关键词： Branch point signal sequences;Peanut;Intron;Molecular markers;Genetic diversity;Fingerprinting

期刊名称：BIOCHEMICAL GENETICS （ 影响因子：1.89； 五年影响因子：1.959 ）

ISSN： 0006-2928

年卷期： 2011 年 49 卷 5-6 期

页码：

收录情况： SCI

摘要： A novel method is introduced for producing molecular markers in plants using single 15- to 18-mer PCR primers designed from the short conserved consensus branch point signal sequences and standard agarose gel electrophoresis. This method was tested on cultivated peanut and verified to give good fingerprinting results in other plant species (mango, banana, and longan). These single primers, designed from relatively conserved branch point signal sequences within gene introns, should be universal across other plant species. The method is rapid, simple, and efficient, and it requires no sequence information of the plant genome of interest. It could be used in conjunction with, or as a substitute for, conventional RAPD or ISSR techniques for applications including genetic diversity analysis, phylogenetic tree construction, and quantitative trait locus mapping. This technique provides a new way to develop molecular markers for assessing genetic diversity of germplasm in diverse species based on conserved branch point signal sequences.

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