The nuclear hormone receptor E75A regulates vitellogenin gene (Al-Vg) expression in the mirid bug Apolygus lucorum

文献类型： 外文期刊

第一作者： Tan, Y. -A.

作者： Tan, Y. -A.;Zhao, X. -D.;Hao, D. -J.;Sun, Y.;Zhao, J.;Jiang, Y. -P.;Bai, L. -X.;Xiao, L. -B.

作者机构：

关键词： Apolygus lucorum;nuclear hormone receptor;20-hydroxyecdysone;vitellogenin;gene regulation

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）

ISSN： 0962-1075

年卷期： 2018 年 27 卷 2 期

页码：

收录情况： SCI

摘要： Apolygus lucorum is the predominant pest of Bacillus thuringiensis (Bt) cotton in China. 20-hydroxyecdysone (20E) plays a key role in the reproduction of this insect. To better understand the mechanism underlying 20E-regulated reproduction, the nuclear hormone receptor E75 isoform-A of Ap.lucorum (Al-E75A) was cloned and its expression analysed. A 2241-bp sequence of Al-E75A cDNA encoded an open reading frame of a polypeptide with a predicted molecular mass of 69.04 kDa. Al-E75A mRNA was detected in female adult stages of Ap.lucorum with peak expression in 7-day-old animals. Al-E75A was also expressed in several tissues, particularly in the fat body and ovary. A 3.2 kb Al-E75A mRNA was detected in all tissues by Northern blot. The fecundity and longevity were significantly decreased in female adults treated with Al-E75A small interfering RNA. The rates of egg incubation rates were considerably lower in the RNA interference-treated animals compared to the untreated controls. In order to investigate the molecular mechanism underlying the effects described above, vitellogenin (Al-Vg) was selected for further investigation. The expression pattern of Al-Vg was similar to that of Al-E75A and was up-regulated by 20E. After knockdown of Al-E75A, the expression profile of Al-Vg and the protein levels were down-regulated. These findings suggest that Al-E75A plays a crucial role in the regulation of Al-Vg expression in Ap.lucorum.

分类号：

  • 相关文献

[1]Sublethal effects of sulfoxaflor on biological characteristics and vitellogenin gene (AlVg) expression in the mirid bug, Apolygus lucorum (Meyer-Dur). Zhen, Congai,Miao, Ling,Gao, Xiwu,Zhen, Congai. 2018

[2]Molecular Cloning and the Expression Profile of Vitellogenin in Relation to Tissue and Food Source in Apolygus lucorum (Hemiptera: Miridae). Yuan, Wei,Zhao, Xincheng,Wu, Kongming.

[3]MOLECULAR CHARACTERIZATION OF SOLUBLE AND MEMBRANE-BOUND TREHALASES IN THE COTTON MIRID BUG, Apolygus lucorum. Tan, Yongan,Xiao, Liubin,Sun, Yang,Zhao, Jing,Bai, Lixin,Xiao, Yingfang.

[4]Molecular characterization and expression of vitellogenin gene from Spodoptera exigua exposed to cadmium stress. Zhao, Jing,Sun, Yang,Xiao, Liubin,Tan, Yongan,Bai, Lixin.

[5]Effect of dietary n-3 LC-PUFAs on plasma vitellogenin, sex steroids, and ovarian steroidogenesis during vitellogenesis in female silver pomfret (Pampus argenteus) broodstock. Peng, Shiming,Gao, Quanxin,Shi, Zhaohong,Zhang, Chenjie,Wang, Jiangang,Yin, Fei,Zhang, Yanliang.

[6]Potential role of D-myo-inositol-3-phosphate synthase and 14-3-3 genes in the crosstalk between Zea mays and Rhizophagus intraradices under drought stress. Li, Tao,Sun, Yuqing,Xu, Lijiiao,Hu, Yajun,Hao, Zhipeng,Zhang, Xin,Li, Hong,Chen, Baodong,Ruan, Yuan,Hu, Yajun,Wang, Youshan,Yang, Liguo.

[7]Gender-specific differences in gene expression profiles in gynogenetic Pengze crucian carp. Zheng, Yao,Chen, Jiazhang,Bing, Xuwen,Yang, Yanping,Zheng, Yao,Chen, Jiazhang,Bing, Xuwen,Yang, Yanping,Zheng, Yao,Chen, Jiazhang,Bing, Xuwen,Yang, Yanping,Zheng, Yao,Yang, Yanping,Wang, Zaizhao,Liang, Hongwei.

[8]Chrysopa septempunctata (Neuroptera: Chrysopidae) Vitellogenin Functions Through Effects on Egg Production and Hatching. Liu, C.,Mao, J.,Zeng, F.,Liu, C..

[9]The tumor suppressor p53 regulates c-Maf and Prox-1 to control lens differentiation. Liu, F. -Y.,Tang, X. -C.,Wu, K.,Wu, M. -X.,Liu, X. -L.,Yu, M. -B.,Liu, Y.,Li, D. W. -C.,Deng, M.,Chen, P.,Ji, W.,Zhang, X.,Gong, L.,Woodward, Z.,Liu, J.,Zhang, L.,Sun, S.,Liu, J. -P.,Li, D. W. -C.,Deng, M.,Deng, M.,Gong, L.,Liu, J.,Zhang, L.,Sun, S.,Li, D. W. -C..

[10]Genome-wide identification and comparative analysis of grafting-responsive mRNA in watermelon grafted onto bottle gourd and squash rootstocks by high-throughput sequencing. Liu, Na,Yang, Jinghua,Fu, Xinxing,Zhang, Li,Guy, Kateta Malangisha,Hu, Zhongyuan,Zhang, Mingfang,Liu, Na,Tang, Kai,Guo, Shaogui,Xu, Yong.

[11]Characterization of a Novel NBS-LRR Gene Involved in Bacterial Blight Resistance in Rice. Wang, Xuming,Chen, Juan,Yang, Yong,Zhou, Jie,Qiu, Yan,Yu, Chulang,Cheng, Ye,Yan, Chengqi,Chen, Jianping,Wang, Xuming,Chen, Juan,Yang, Yong,Zhou, Jie,Qiu, Yan,Yu, Chulang,Cheng, Ye,Yan, Chengqi,Chen, Jianping,Wang, Xuming,Chen, Juan,Yang, Yong,Zhou, Jie,Qiu, Yan,Yu, Chulang,Cheng, Ye,Yan, Chengqi,Chen, Jianping.

[12]Divergently expressed gene identification and interaction prediction of long noncoding RNA and mRNA involved in duck reproduction. Ren, Jindong,Hu, Jianhong,Ren, Jindong,Du, Xue,Zeng, Tao,Chen, Li,Shen, Junda,Lu, Lizhi.

[13]Enhanced effects by mixtures of three estrogenic compounds at environmentally relevant levels on development of Chinese rare minnow (Gobiocypris rarus). Yang, Fangxing,Jin, Shiwei,Jin, Shiwei,Hui, Yang,Xu, Ying,Liao, Tao,Wen, Sheng.

[14]Comparative responses in rare minnow exposed to 17 beta-estradiol during different life stages. Liao, T.,Guo, Q. L.,Xu, Y.,Liao, T.,Cheng, W.,Jin, S. W.. 2009

[15]The Discrepant and Similar Responses of Genome-Wide Transcriptional Profiles between Drought and Cold Stresses in Cassava. Zeng, Changying,Ding, Zehong,Zhou, Fang,Zhou, Yufei,Yang, Ruiju,Yang, Zi,Wang, Wenquan,Peng, Ming. 2017

[16]MicroRNA-275 and its target Vitellogenin-2 are crucial in ovary development and blood digestion of Haemaphysalis longicornis. Hao, Jiawei,Luo, Jin,Chen, Ze,Ren, Qiaoyun,Guo, Jinxia,Liu, Xiaocui,Chen, Qiuyu,Wu, Feng,Wang, Zhen,Luo, Jianxun,Yin, Hong,Wang, Hui,Liu, Guangyuan,Yin, Hong,Wang, Hui,Wang, Hui. 2017

[17]Characterization of a pattern recognition molecule vitellogenin from carp (Cyprinus carpio). Liu, Qing-Hui,Gao, Chun-Ren,Zhang, Shi-Cui,Li, Zhao-Jie. 2009

[18]Vitellogenin and vitellogenin receptor gene expression profiles in Spodoptera exigua are related to host plant suitability. Zhao, Jing,Sun, Yang,Xiao, Liubin,Tan, Yongan,Jiang, Yiping,Bai, Lixin,Zhao, Jing. 2018

[19]The dynamic landscape of gene regulation during Bombyx mori oogenesis. Zhang, Qiang,Sun, Wei,Sun, Bang-Yong,Zhang, Ze,Xiao, Yang,Zhang, Ze. 2017

[20]Down-regulation of the OsPDCD5 gene induced photoperiod-sensitive male sterility in rice. Wang, Yufeng,Zha, Xiaojun,Qian, Xiaoyin,Dong, Xianxin,Sun, Fan,Yang, Jinshui,Zhang, Shiyong. 2010

作者其他论文 更多>>

微信小程序